Page 61 - AQ242MP WW NO-CAL-2242-01-0519-water seminar

INDEPENDENT LAB REPORTS RESULTS

Rena Ware at home filtration systems

The Rena Ware Aqua Nano at home water filters have undergone thorough biological testing provided by South
Korea-based laboratory NoroGene Research Center. Below are the results.

Approximate Approximate MS2 Bacteriophage (virus), PFU/mL Raoultella Terrigena (bacteria), PFU/mL
Volume of Water Volume of Water Influent, Effluent, % Influent, Effluent, %
(Gallons) (Liters) PFU/mL PFU/mL Log Reduction PFU/mL PFU/mL Log Reduction

360 1362.75 47,000 0.5 4.67 99.9989% 720,000 <0.5 5.86 >99.9993%
1440 5450.99 82,000 8 4.01 99.9902% 450,000 <0.5 5.66 >99.99989%

2520 9539.23 72,000 39 3.27 99.9458% 310,000 <0.5 5.49 >99.99984%
3600 13627.48 92,000 73 3.10 99.9207% 640,000 <0.5 5.81 >99.99992%
Average 3.76 99.9639% Average 5.71 99.99990%

Minimum of volume (gallons) of dechlorinated tap water passed through each filter. The flow rate was maintained at
approximately 1-1.3 gallon/minute at a pressure of 50-60 PSI. Ten liters of deionized lab grade water was seeded
with the indicated microorganisms and passed through the filter at a flow rate of 1.0-1.2 gallon/minute at each
of the test points indicated. Filter influent and effluent following the passage was collected and assayed for the
respective microorganisms.

The following are some common microorganisms that form part of the group of bacteria and viruses and the diseases
they are related to:
Bacterias: Vibrio Cholerae (Cholera), Samonella Typhi (Typhoid fever), Helicobacter Pylori (H. pylori infection),
Shigella (Shigellosis).
Viruses: Norwalk (Viral Gastroenteritis), Hepatitis A (Hepatitis).

Rena Ware Filter Bottle

The Rena Ware Filter Bottle has undergone thorough biological testing provided by Biological Consulting Services
(BCS) of North Florida, Inc. Below are the results.

Rena Ware 1600 L Challenge 400 ml/min Raoutella Terrigena (RT), MS-2, Filtration Efficacy Study;
General Test Water 1 (GTW1, Dechlorinated Municipal Water)

Presure Influent's Effluent's Calculated Influent's Effluent's Calculated
Client ID BCS ID at Flowrate RT Bacteria RT Bacteria Bacteria MS-2 Average MS-2 Average MS-2 Virus
Challenge (mL/min) Concentration Concentration Percent Concentration Concentration Percent
(PSI) (cfu/mL) (cfu/mL) Removal (pfu/mL) (pfu/mL) Removal
Rena Ware
Unit 3 1807167 -2.3 400.0 3.4 x 10 5 27.7 99.992% 2.9 x 10 5 5.0 E + 03 98.3 %

The received bottles unit was conditioned by soaking the filter in General Test Water 1 (GTW1(NSF P231), Dechlorinated Municipal water) and then aspirating
1-Liter at a flow rate of 400 mL/min using a peristaltic pump as per NSF guidance. Silicone tubing was connected to a programmable peristaltic pump and
was placed onto the mouth suction tab of the bottle. The entirety of the bottle unit was submerged in the GTW1 (the bottom of the bottle was unscrewed and
a vent hole was placed into the upper side of the plastic bottle about 1" below the threadline to facilitate bottle filling and submersion, additionally one was
also placed in the bottom of the bottle). The pump was activated and the flow rate gradually was increased from 100 mL/min to 400 mL/min. The line vacuum
was measured continuously and did not exceed 1.0 PSI. For the challenge, aliquots of R. terrigena (RT) culture and MS-2 virus were added to GTW1 and the
water was homogenized. A sample of the challenge water was removed and enumerated for the respective microorganisms prior to challenge initiation. 500ml
of challenge water was aspirated through the unit at a flowrate of 400mL/min and the resulting vacuum. During the challenge the water level was maintained
at height below the top orifice of the filter unit; this was done to eliminate the possibility of the challenge water bypassing the filter unit. Following the initial
challenge, the bottle unit was submerged in GTW1 and 600L was aspirated through the unit at 400mL per minute. Following 800L, 1200L and 1600L, the
challenge was repeated. The line vacuum was monitored and did increase above what was initially measured at the lower flow rate. For each of the challenge
events, the unit's collected influent and effluent samples were analyzed in duplicates at two dilutions at the minimum as per standard lab operating procedure.

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