Page 183 - pathology_services_handbook_5th_edition_2018

Procedure of Collection

1. Write the patient’s name, RN/IC and test requested on the label of the container
(If collection is done by the patient, the laboratory staff will write down the
patient’s detail as written on the request form). Once collection is started, all
urine excreted within the time should be collected in the container.
2. On the first day of collection, the first urine voided must be discarded. Time of CHEMICAL PATHOLOGY
first urine voided is the start of the timing for the 24 hours collection. Write the
date and time on the label.
3. Thereafter, all urine should be collected until the end of the 24 hours period.
4. Urine should be passed into a separate small container at each voiding and then
emptied into the 24 hours urine container. Do not urinate directly into the 24
hours urine container to avoid any injury cause by the content preservative (e.g.
acid).
5. If the patient has a bowel movement during the collection period, precautions
should be taken to prevent faecal contamination.
6. At the end of the collection, the last urine voided is collected. The collection is
now complete.
7. For in-patient, the ward staff must follow these insructions:
• Measure and record the 24 hours urine volume on the test request form and
patient’s record.
• Mix the contents of the 24 hours urine container gently but thoroughly.
• Examine to ensure that the contents appear homogeneous.
• Transfer at least 10mL of urine from the 24 hours urine container into a
universal container.
• Label the universal container with patient’s name, RN/IC and test requested.
8. If collection is done by the patient, send the entire 24 hours urine sample with
request form to the laboratory.

Notes:
Ensure the container cap is firmly screwed before transportation to prevent leakage.
Refrigerate the sample for the best result.

4.2 Creatinine Clearance Test

1. Follow Procedure 4.1 for 24 hours urine collection.
2. During the 24 hours urine collection time (but not within 1-3 hours after a large
meal), send a blood sample for serum creatinine analysis.

Notes:
Serum creatinine level during the 24 hours urine collection is required to enable the
calculation of creatinine clearance.
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