Page 204 - pathology_services_handbook_5th_edition_2018

2.4 Indwelling devices

HTAA, KUANTAN Catheters for semi-quantitative culture eg: Hickman catheter, Broviac catheter, arterial,
The acceptable devices are:

umbilical, central line and Swan-Ganz. Foley’s catheter is not acceptable for culture.

1. Clean the skin around the catheter site with alcohol. Let it air dry.
2. Aseptically remove catheter and cut about 5 cm of the distal tip of the catheter
using sterile cutter and directly place into a sterile screw-cap container.
3. Transport directly to the laboratory to prevent drying.

2.5 Mycobacterium: Acid-fast bacilli stain and culture and MTBC & rifampicin
resistance detection by using Gene Xpert MTB/Rif assay

A. Acid-fast bacilli stain

Acceptable specimens:

Sputum/respiratory secretion, bronchial wash, urine, CSF, body fluid/aspirate, gastric
lavage, tissue biopsies and FNAC specimen. Swab and stool are not acceptable.

1. Collect at least sputum samples with at least 1 early morning sputum.
2. Place specimens in sterile container and send to the laboratory.

B. Mycobacterium culture

Acceptable specimens:

Sputum/respiratory secretion, bronchial wash, urine, CSF, body fluid/aspirate, gastric
lavage, tissue biopsies, bone marrow and blood. Swab and stool are not acceptable for
culture.

1. Collect specimen and place in an appropriate container i.e bone marrow and
blood in Myco/F lytic bottle and other specimens in sterile container.
2. Send specimen to the laboratory.

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