Page 75 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

The efficiency of inhibition the growth of weeds by allelopathy extract from
Little ironweed in different solvents

Kasidit Luecha , Manatsanan Kaewmala , Wannida Chueasuriya
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Advisor: Suwanna Thima
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Princess Chulabhorn Science High School Chiang Rai
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Abstract
The objectives of this project was to compare the efficiency of weed eliminating allelopathy extracts
from Little Ironweed (Vermonia cinerea (L.) Less.) in different solvents. This project was conducted in
2 steps. Step 1, the extraction of allelopathy from Little Ironweed (Vermonia cinerea (L.) Less.) with
solvents which are distilled in water, Hexane and Ethyl Acetate in the concentration of 1:10 ml, 1:20
ml, and 1:30 ml (extract: solvent). The result showed that allelopathy in Little Ironweed was the greatest
extract by distilled water, followed by Hexane and Ethyl Acetate. Step 2, tested the extraction of
allelopathy from Little Ironweed (Vermonia cinerea (L.) Less.) by taking the extract to test the inhibition
of seed germination and see the growing progress. There were two kinds of seed in this experiment
which are Gold Beard Grass (Vermonia cinerea (L.) Less.) and Sensitive Plant (Mimosa pudica L.) Two
kinds of seeds were soaked in the extract that had different concentrations for 12 hours. After that,
researchers planted the weed seeds that had been soaked on Petri dishes that had 10 mg of magic crystal
soil and then added 20 weed seeds per dish to find the percentage of inhibiting germination and left the
dish under fluorescent light for 8 hours per day for 14 days. The result showed that the extract from
Little Ironweed (Vermonia cinerea (L.) Less.) with distilled water in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 73.38%Secondly, the extract
from Little Ironweed(Vermonia cinerea (L.) Less.) with Hexane in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 64.45%.Lastly, the extract from
Little Ironweed(Vermonia cinerea (L.) Less.) with Ethyl Acetate in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 54.09%

Keywords: Allelopathy substance, Vermonia cinerea (L.) Less., Mimosa pudica L., Vermonia cinerea
(L.) Less., distilled water, Hexane and Ethyl Acetate

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