Page 25 - Clinical Hematology Atlas

CHAPTER 1 INTRODUCTION TO PERIPHERAL BLOOD SMEAR EXAMINATION 9

TABLE 1-1 Cells Found in a Normal White Blood Cell Differential

Adult Reference Range
Cell
Size Peripheral Cells 3
Cell Type (mm) Nucleus Chromatin Cytoplasm Granules Blood (%) 10 /L
9
Segmented 10-15 2-5 lobes Coarsely Pale pink, 1°: Rare 50-70 2.3-8.1
neutrophil (Seg), connected by clumped cream 2°: Abundant
polymorphonuclear thin filaments colored, or
neutrophil (Poly, without colorless
PMN) visible
chromatin

Band neutrophil 10-15 Constricted, Coarsely Pale blue to 1°: Few 0-5 0.0-0.6
(Band) but chromatin clumped pink 2°: Abundant
must be
visible within
the thinnest
part

Lymphocyte 7-18* Round to Condensed Scant to mod- 6 Few azurophilic 20-40 0.8-4.8
(Lymph) oval; may be to deeply erate; sky blue
slightly condensed
indented;
occasional
nucleoli

Monocyte (Mono) 12-20 Variable; may Moderately Blue-gray; Many fine granules, 3-11 0.5 to 1.3
be round, clumped; may have frequently giving the
horseshoe, lacy pseudopods; appearance of ground
or kidney vacuoles may glass
shaped; often be absent or
has folds numerous
producing
“brainlike”
convolutions
Eosinophil (Eos) 12-17 2-3 lobes Coarsely Cream to 1°: Rare 0-5 0.0-0.4
connected clumped pink; may 2°: Abundant red to
by thin have irregular orange, round
filaments borders
without
visible
chromatin
Basophil (Baso) 10-14 Usually Coarsely Lavender to 1°: Rare 0-1 0.0-0.1
two lobes clumped colorless 2°: Lavender to, dark
connected purple; variable in
by thin number with uneven
filaments distribution; may
without obscure nucleus or
visible wash out during
chromatin staining, giving the
appearance of empty
areas in cytoplasm

*The difference in size from small to large lymphocyte is primarily due to a larger amount of cytoplasm. See Chapter 9 for more detailed information on
lymphocyte size.
1⁰ 5 primary, 2⁰ 5 secondary.