Page 97 - Clinical Hematology Atlas

CHAPTER 8 BASOPHIL MATURATION 81

BASOPHIL

A B
FIGURE 8–2A Basophil. FIGURE 8–2B Basophil. Note that granules are
water-soluble and may be dissolved during the
staining process, leaving clear area in the
cytoplasm.

SIZE: 10-14 mm
NUCLEUS: Usually two lobes connected by thin
filaments without visible chromatin
Nucleoli: Not visible
Chromatin: Coarse, clumped
CYTOPLASM: Lavender to colorless
Granules:
Primary: Rare
Secondary: Variable in number with uneven
distribution, may obscure nucleus
(Figure 8-2A); deep purple to black;
irregularly shaped. Granules are water-
soluble and may be washed out during
staining; thus they appear as empty areas C
in the cytoplasm (Figure 8-2B). FIGURE 8–2C Electron micrograph of basophil
N/C RATIO: Cytoplasm predominates (328,750).
REFERENCE INTERVAL:
Bone Marrow: ,1%
Peripheral Blood: 0% to 1%

Refer to Table 1-1 for more examples.

All photomicrographs are 31000 with Wright-Giemsa stain unless stated otherwise.