Page 4 - Module & More Biology F4

Biologi Tingkatan 4 Bab 9 Nutrisi dan Sistem Pencernaan Manusia

Pencernaan kanji, protein dan lipid dalam sampel makanan
Eksperimen 9.1 The digestion of starch, protein and lipid in food samples

TP 3
1. Kanji / Starch

Tujuan Mengkaji tindakan amilase liur dalam pencernaan sampel makanan berkanji.
Aim To study the action of salivary amylase in the digestion of starchy food samples.
Pernyataan
masalah Apakah kesan tindakan enzim amilase liur terhadap kanji?
Problem What is the action of salivary amylase on starch?
statement
Hipotesis Enzim amilase liur menghidrolisis kanji kepada gula penurun.
Hypothesis The salivary amylase hydrolyses starch into reducing sugar.
Pemboleh (a) Dimanipulasikan: kehadiran amilase liur
ubah Manipulated: absence or presence of salivary amylase
Variables (b) Bergerak balas: kehadiran gula penurun
Responding: presence of reducing sugar
(c) Dimalarkan: kepekatan larutan kanji, suhu, isi padu larutan kanji
Constant: starch concentration, temperature, volume of starch

Bahan Air liur, ampaian kanji 1%, larutan iodin, larutan Benedict, air suling
Materials Saliva, 1% starch suspension, iodine solution, Benedict's solution, distilled water

Radas Bikar, tabung uji, silinder penyukat (5 ml), jam randik, pemegang tabung uji, kukus air, termometer, penitis,
Apparatus rod kaca
Beakers, test tubes, measuring cylinders (5 ml), stopwatch, test tube holders, water bath, thermometers, droppers, glass rod
9 Prosedur 1. Kumur mulut dengan air suling dan kumpul air liur (2 ml) dalam sebuah bikar.
Procedure
Rinse the mouth with distilled water and collect saliva (2 ml) in a beaker.

2. Cairkan air liur dengan 2 ml air suling.
Add 2 ml of distilled water to the saliva.
BAB 3. Sediakan 2 tabung uji berlabel A dan B seperti dalam jadual.
Prepare 2 test tubes labelled A and B as shown in the table.

Tabung Uji / Test tube Kandungan / Contents

1 ml larutan air liur + 2 ml larutan kanji
A
1 ml saliva solution + 2 ml starch solution
B 1 ml air suling + 2 ml larutan kanji
1 ml distilled water + 2 ml starch solution
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4. Simpan semua tabung uji tersebut dalam kukus air 37 C selama 10 minit.
Keep the test tubes in water bath at 37 C for 10 minutes.
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5. Uji kandungan setiap tabung uji dengan ujian iodin dan ujian Benedict.
Test the contents of each test tube with iodine test and Benedict’s test
6. Catat keputusan ujian.
Record the results.
Pemerhatian Tabung Uji / Test tube Ujian iodin / Iodine test Ujian Benedict / Benedict's test
Observation
Kekuningan Mendakan merah bata
A
Yellowish Brick red precipitate
B Biru hitam / Dark blue Biru / blue

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