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Production of 2,3 butanediol and succinic acid from molasses as a

monomer for 2,3-BDO-co-1,4-BDO-co-succinate (PBBS) syntheses

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Prawit Kongjan , Nantharat Phruksaphithak , Sureewan Sittijunda 3,*

1 Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
2 Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung 93110, Thailand
3 Faculty of Environment and Resource Studies, Mahidol University, Nakhon Pathom 73170, Thailand
*Corresponding author: Sureewan.sit@mahidol.ac.th
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Abstract

This research aims to produce 2,3 butanediol (2,3-BDO) and succinic acid as monomers
for 2,3-BDO-co-1,4-BDO-co-succinate (PBBS) syntheses. First, the extraction and purification of

2,3-BDO from the fermentation broth on the pilot scale were investigated. The 2,3-BDO
production of 5.46 ± 0.24 g/L was obtained using 20 g-VS/L of molasses as the substrate and

Enterobacter sp. MU-01 as inoculum. The optimal conditions for extraction and purification of
2,3-BDO from the fermentation broth were butyraldehyde (BA) and fermentation broth and
PDD: H O ratios of 1:1 and 1:1, respectively. Under these conditions, up to 53 % of 2.3-BDO
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can be extracted, and 68.3 % of BA can be recovered. Next, the isolation, characterization,
and optimization of succinic acid production from molasses were examined. The result

showed that the isolated strain was Enterococcus sp. MUSA001. The optimal condition for
succinic acid production was 60 g/L of molasses, 0.85 g/L of lactic acid as electron donor, 29.91

g/L Na CO , an initial pH of 5.0, with a maximum succinic acid production of 0.68 g/L. Last,
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PBBS synthesis uses commercial monomers (2,3-BDO, 1,4-BDO, and succinic acid) as a model
was conducted. The result showed that the suitable condition for synthesizing PBBS with a
molecular weight greater than 100,000 g/mol was succinic acid 1,4-BDO/2,3-BDO and catalyst

of 100:200(200:100):1 (molar ratio), the temperature of 200 ºC and reaction time of 36 h,
respectively.

Keywords: 2,3-butanediol, succinic acid, 2,3-BDO-co-1,4-BDO-co-succinate, molasses