Page 153 - KOMPILASI ARAHAN TUGASAN PEKA SPM KINTA UTARA 2022

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1. Dua piring petri yang mengandungi 10 cm agar-agar nutrient steril disediakan dan dilabelkan A dan B
seperti rajah di atas.
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Two petri dishes containing 10 cm of sterile nutrient agar were prepared and labeled A and B as in the
figure above.
2. Piring petri A dipanaskan di dalam ketuhar sehingga kering. Piring petri A dikeluarkan dari ketuhar
untuk disejukkan pada suhu bilik.
Petri dish A is heated in the oven until dry. A petri dish is removed from the oven to be cooled to room
temperature.
3. Hujung dawai gelung disterilkan dengan memanaskannya dalam nyalaan penunu Bunsen sehingga
dawai gelung membara.
The end of the loop wire is sterilized by heating it in a Bunsen burner flame until the loop wire is burning.
4. Selepas hujung dawai gelung disteril, hujung dawai gelung dialihkan daripada nyalaan penunu Bunsen
PANITIA SAINS DAERAH KINTA UTARA
dan dawai gelung dibiarkan menyejuk pada suhu bilik.
After the end of the loop wire is sterilized, the end of the loop wire is removed from the Bunsen burner
flame and the loop wire is allowed to cool to room temperature.
5. Hujung dawai gelung dicelupkan ke dalam larutan kultur bakteria Bacillus sp.
The end of the loop wire is dipped into a solution of Bacillus sp.
6. Hujung dawai gelung digunakan untuk melumurkan kultur bacteria secara zigzag ke atas agar-agar
nutrient steril di dalam piring petri A.
The end of the loop wire is used to smear the bacterial culture in a zigzag pattern on the sterile nutrient
agar in petri dish A.
7. Langkah 2 hingga 5 diulangi dengan piring petri B.
Steps 2 to 5 are repeated with petri dish B.
8. Piring petri A dan B ditutup dan penutup dilekatkan dengan pita selofan.
Petri dishes A and B are closed and the lids are sealed with cellophane tape.
9. Piring petri disimpan secara terbalik di dalam almari yang gelap pada suhu bilik selama tiga hari.

Petri dishes are stored upside down in a dark cupboard at room temperature for
three days.

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