Page 335 - Catalogue 2015

TG Buffer 10x concentrate

Synonym: Tris-Glycine buffer
Composition: Tris Base 0.25M, Glycine 1.92M.

D 1.063;

EUH:210;

Cat. No. TG Buffer 10x concentrate
203123
Molecular biology
Application:
Commonly used for non-denaturing polyacrylamide gel electrophoresis of proteinsand for
electro-blotting proteins in Western blot procedures. Also recommended for use with all
nitrocellulose and nylon membranes.

Appearance Clear colorless liquid RNase activity Not detected
pH of 10X Conc. (25°C) 8.4-8.8 Protease activity Not detected
Conductivity of 10X Conc. (25°C) 3-5mS/cm
DNase activity Not detected

TG-SDS Buffer 1X

Synonym: Tris-Glycine-SDS buffer
Composition: TRIS base 0.025M, Glycine 0.192M, Sodium dodecyl sulfate (SDS) 0.1%

D 1.0;

Cat. No. TG-SDS Buffer 1X
203023 Molecular biology

Application: Commonly used as a running buffer in SDS-PAGE.

Appearance Clear colorless liquid RNase activity Not detected
pH 8.4-8.8 Protease activity Not detected
DNase activity Not detected

TG-SDS Buffer 10X

Synonym: Tris-Glycine-SDS buffer
Composition: TRIS base 0.25M, Glycine 1.92M, Sodium dodecyl sulfate (SDS) 1%

D 1.07;

EUH:210;

Cat. No. TG-SDS Buffer 10X
205223 Molecular biology

Application: Commonly used as a running buffer in SDS-PAGE.

Appearance Clear colorless liquid RNase activity Not detected
pH of 1X Conc. (25°C) 8.4-8.8 Protease activity Not detected
pH of 10X Conc. (25°C) 8.4-8.8
DNase activity Not detected