Page 44 - Catalogue 2015

SOLVENTS FOR ULC/MS – CC/SFC

Biosolve early recognized the importance of high purity solvents, formulations and reagents for
sophisticated LC and LC-MS applications and began offering a decade ago the ULC/MS grade which is
the finest grade available anywhere in the world for UHPLC/ MS applications. Biosolve Ultra Liquid
Chromatography / Mass Spectrometry (ULC/MS) solvents for high resolution and sensitivity checked and
guaranteed to meet the demand for high chemical purity, high UV transmission and lowest peak impurities
in the MS and PDA gradient elution tests to ensure reproducibility.

Latest innovations in convergence chromatography (CC), the supercritical fluid chromatography (SFC) led
us testing our ULC/MS grade products also for CC/SFC application. Supported by significant investment
in R&D, the results show that the ULC/MS grade products have the highest chemical purity, superior
performance for MS applications, excellent UV transmission and the lowest peak impurities under gradient
conditions including with CC/SFC application. As a result, and for the convenience of our customers we
have added the term CC/SFC to our finest ULC/MS grade products, which are now called ULC/MS-
CC/SFC.

Biosolve investment in this research demonstrates our commitment to constantly improving our products
and our abilities to meet your new requirements by remaining a leader manufacturer in our field.
All ULC/MS-CC/SFC solvents are filtered at 0.1μm and packed under inert gas for better shelf life.
Standard packages are available in 1L, 2.5L, PTFE lined amber bottles and for some solvents stainless
steel containers are available that can be equipped with dip pipe delivery devices from 25L to 1500L
volume.

PDA gradient between 200-400 nm of Acetonitrile : Water
ULC/MS grade. Loading for 15 min. gradient 10-100%
Acetonitrile in 20 min., hold 5 min. Column RP18, 3.5um; flow
1 ml/min.

Comparison between the positive ESI spectra of Acetonitrile,
MeOH & Water ULC/MS grade versus Reserpine 10ppb at
100-1000 m/z