Page 87 - Catalogue 2015

BES free Acid

Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)tauri

CAS [10191-18-1]; EC 233-465-5; CÃHÌÂNOÂS; M 213.25

Warning; H:315-319-335; P:261-280-305+351+338-321

Cat. No. BES free Acid
023223 Molecular biology

Application: Commonly used for preparation of buffered saline for transfection of DNA into mammalian cells.
pKa =7.1 at 25°C; useful pH range 6.4 - 7.8.

Appearance White powder A260nm (10%) max. 0.1AU
Solubility (10% in Water) Clear colorless solution A280nm (10%) max. 0.1AU
Loss on drying (105°C) max. 0.5% DNase activity Not detected
pH (10% in Water) 3.5-5 RNase activity Not detected
Assay (T) min. 99%w/w Protease activity Not detected

Betaine 5M

Synonym: (Carboxymethyl)trimethylammonium inner salt, Oxyneurine

CAS [107-43-7]; CÂHÌÌNO¿; M 117.15;

Warning; H:302; P:264-270-301+312-330

Cat. No. Betaine 5M
026557 PCR reagent

Appearance Clear solution RNase activity Not detected
Assay (T) 4.8-5.2M Protease activity Not detected
DNase activity Not detected

Bicine

Synonym: N,N-Bis(2-hydroxyethyl)glycine

CAS [150-25-4]; EC 205-755-1; CÃHÌÀNOÁ; M 163.17

Cat. No. Bicine
023323 Molecular biology

Application: Commonly used as buffering substance useful at pH range 7.6 - 9.0, pKa = 8.26 at 25°C.

Appearance White powder A260nm (1M) max. 0.1AU
Solubility (1M in water) Clear colorless solution A280nm (1M) max. 0.1AU
Loss on drying (105°C) max. 0.5% DNase activity Not detected
Heavy metals (as Pb) max. 0.001% RNase activity Not detected
pH (1M in water) 3.5-5 Protease activity Not detected
Assay (T) min. 98.5%w/w