Page 210 - 2022-23 BIOFACT Catalogue
P. 210

HiGene™


                  Pre-made buffer


                  Convenient Solution for Molecular Biology and Biochemical Applications






                  HiGene™ Pre-made bu er is designed for convenient use and it is suitable for molecular biology and biochemical applications
                  such as nucleic acid gel electrophoresis, blotting, and DNA extraction.

                    Product Information
                    HiGene™ Pre-made bu er























                                       Bu er composition  Bu er size   Storage temp.  How to use
                       HiGene™
                      50X TAE bu er  2M Tris-Acetate, 50 mM EDTA  1 L  RT      Dilute 50X bu er to 1X or 0.5X.
                       [PB101-10h]
                       HiGene™      445mM Tris, 445mM Boric Acid,             Put it in a gel tank and use it or use
                      5X TBE bu er     10mM EDTA pH8.0   1 L      RT             it when making gel.
                       [PB102-10h]
                        HiGene™
                    RNase Free water (DEPC)  Diethyl pyrocarbonate (DEPC) 0.1%  1 ㎖ x 81 ea  RT  RT-PCR & RNA sample preparation, etc.
                    [PB141-081,PB141-B500]              / 500 ㎖
                     HiGene™ 0.5M EDTA   0.5M EDTA      500 ㎖     RT            Dilute it at the appropriate
                       [PB131-500]                                              concentration and use it.
                        HiGene™
                    10X SDS PAGE running   250mM Tris-HCl (pH8.3),              Dilute 10X buffer to 1X and
                        bu er         1.92M glycine, 1% SDS  1 L  RT            use it for SDS gel loading.
                       [PB151-10h]
                        HiGene™
                    Coomassie blue staining   50% Methanol, 0.05% Coomassie
                        solution   brilliant blue R-250, 10% Acetic acid  1 L  RT  Use it for SDS-PAGE gel stain.
                       [PB152-10h]
                        HiGene™
                    Coomassie blue destain-  45% Methanol, 10% Acetic acid  1 L  RT  Use it for SDS-PAGE gel stain.
                       ing solution
                       [PB153-10h]



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