Page 232 - 2022-23 BIOFACT Catalogue

Feature of various membrane materials in syringe lter
CA PES MCE Nylon PVDF PTFE-W PTFE-O
Sterilized Sterilized Sterilized Non-sterilized Non-sterilized Non-sterilized Non-sterilized
Hydrophilic Hydrophilic & Hydrophobic Hydrophobic
Low protein binding High protein binding Low protein binding

Clarifying ltration,
Particle removal Filtration of culture Pre- ltration of Filtration of organic
Filtration of
ltration of biochem- medium, additive HPLC sample ltration aqueous solution Filtration of mild- mixed solvent used solvent / HPLC
oganic solvent
and particle removal
ical solution and bu er in HPLC sample
ltration

Sample volume

13 ㎜ for up to 10 ㎖ 25 ㎜ for up to 100 ㎖
Available Volume : 0.3 – 10 ㎖ Available Volume : 10 – 100 ㎖

＊Filtration capacity may be di erent on various solution type, character of solution or pH.

Feature of two pore sizes in syringe lter

Sterilization Particle Removal
0.2 ㎛ 0.45 ㎛
(Columns packed < 3 ㎛ particles) (Columns packed > 3 ㎛ particles)
Bacteria, UHPLC, etc. Particles, HPLC, etc.

Application notes of syringe lter
Type of Filtration Recommended Alternatives
HPLC · UHPLC · LC/MS · GC (Sample pretreatment) PTFE PVDF / Nylon
Undiluted Organic Solvents PTFE PVDF / Nylon
Protein Analysis · Samples with Biomolecules-Bu er PES CA / MCE / PVDF
Tissue Culture Media PES CA
High Particle-load Sample – Organic Solvents Hydrophobic PTFE-O PTFE / PVDF
High Particle-load Sample – Aqueous Solvents Nylon -

232 BIOFACT CATALOGUE