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i. FBP is NOT indicated in the following:
• Healthy patient with normal blood cell indices including medical check-up
except personnel exposed to radiation.
• Post transfusion sample except as part of investigation of transfusion reaction.
• Investigation of anaemia with clinical evidence of recent bleed.
j. It is compulsory to write relevant clinical information ( clinical summary and
indication for FBP request) in the request form. HAEMATOLOGY
k. FBP urgent : If FBP is really needed urgently, please state reasons and you should
contact/see medical officer or pathologist in charge.

4.4 Osmotic Fragility Test, CD4/CD8 Count, Sickling test, Kleihauer test and Neutrophil
Alkaline Phosphatase score
All these test require appointment. On the appointment date, collect the blood
specimen in the EDTA tube and send to the laboratory immediately.

4.5 Coagulation and Hemostasis test (PT, aPTT, fibrinogen, D-dimer, mixing test,
factor assays, lupus anticoagulant, inhibitor study).

a. For haemostasis test venous blood sample should be obtained by clean
venepuncture at a site away from an intravenous line. This is important as
heparin/ other contamination will cause spurious abnormal result.
b. During blood collection, use light pressure using a tourniquet and avoid
prolonged application (if possible < 1 minute). Avoid slow flowing draws and/or
traumatic venepunctures.
c. Use sodium Citrate tube container and it must be adequately filled (follow the
ring mark on the tube).
d. Sample should be mixed thoroughly with the anticoagulant by gentle inversion
of the blood tube.
e. The sample must be transported to the laboratory as soon as possible and
processed (centrifuged)/tested within 3 hours after sampling.
f. Request for D-dimer, mixing test, factor assays and inhibitor are on appointment
basis. Please call medical officer on call for approval.

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