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Important information to be included in the request form are:
• Creatinine kinase level
• LDH
• Contact number of treating clinician relevant hospital fax number

For open muscle biopsy: HISTOPATHOLOGY
• Remove at least one good cylinder of muscle measuring 1.0 x 0.5 x 0.5 mm, the
smallest diameter being the cross-section of the muscle.
• For optimal processing muscle specimen must be wrapped with aluminium foil
and sent fresh in and air tight, dry and clean container.
• The specimen should be transported in a container containing ice or gel ice
(Never use dry ice).
• Do not tie the muscle to stretch it and do not put tissue on gauze.
• Do not put saline or formalin. The laboratory will accept fresh muscle biopsy
specimen up to 6 hours upon removal but please remember that optimal result
requires the specimen to be as fresh as possible.

5.0 SPECIMEN TRANSPORTATION

a. Specimens from the Operation Theatre (OT) should be sent to Histopathology
laboratory before 4:00 pm if possible. Any specimens obtained after 4:00 pm
should be sent on the following day.
b. Specimens from the clinics should be sent immediately to the Histopathology
laboratory.
c. The MLTs who receive the specimens and the HPE request forms must sign the
despatch book.

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