Page 224 - pathology_services_handbook_5th_edition_2018

d. Request for frozen section must be made via appointment with Histopathologist
on call and followed by filling the REQUEST FORM FOR INTRAOPERATIVE
HTAA, KUANTAN e. On the day of operation, the fresh specimen should be labeled and accompanied
CONSULTATION / FROZEN SECTION (ANA/JP/HTAA-06) and sending the form to
Histopathology Laboratory at least a day before operation.

by the request form i.e. PERMOHONAN PATOLOGI ANATOMI ANA/JP/HTAA-01
(2 copies) before sending to the laboratory.

4.0 SPECIMEN COLLECTION

a. All tissue specimens for routine histological examination must be preserved
immediately in formalin, i.e 10% Neutral Buffered Formalin (NBF) in a suitable
clean leak proof container.
b. Tissue should be totally submerged in formalin. The volume of formalin should
be at least 10 times the volume of the specimen.
c. The containers used should be of the wide mouthed type for easy removal of
tissue.
d. The tissue container should be appropriate according to the size of the tissue.
Do not put the tissue in plastic bag to prevent leakage unless the tissue is too
big.
e. Screwed cap container/universal bottle should be used for small and tiny tissue
biopsies.
f. All specimen containers should have the same identification details as that
written on the request forms.
g. Specimen for frozen section should be collected fresh and send immediately in
clean empty universal container without any solution.
h. Renal biopsy should be collected in three tissue containers as below :
• One with Phosphate-buffered Saline (PBS) for immunofluorescence test
• One with 10% formalin for routine histology. Do not put specimen in gauze!
• One with glutaraldehyde for Electron Microscopy (EM) study. However, this
test will be done in EM Unit, IIUM Kuantan Pahang.
i. Skin biopsy for immunofluorescence test should be collected in tissue container
containing Phosphate-buffered Saline (PBS).
j. Muscle biopsy (this test will be outsourced to Hospital Kuala Lumpur)
• All requests for muscle biopsy must have prior arrangement with the
patologist concerned or Medical Lab Technologist (MLT) Hospital Kuala
Lumpur at extension 5605 at least one day before the procedure. (Usually
the biceps or quadriceps muscle would suffice in most cases).

214