Page 235 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

CHAPTER 10 ■ Basic Laboratory Assessment of Erythrocytes, Leukocytes, and Platelets 219

TABLE 10.4 Observations at Various Microscopic Magni cations

Low Power (10×) High Power (40×) Oil Immersion (100×)

Erythrocytes Rouleaux, agglutination Locate appropriate examination Evaluate size, shape, and color and inclusion

area for 100× bodies

Leukocytes Scan for abnormal cells Determine estimate of total WBC Evaluate and differentiate 100 WBCs; observe

such as smudge cells count any inclusions or other abnormalities

Platelets Scan for clumps and Determine estimate of total platelet count

satellitism Evaluate platelet size and granularity

Microscopic Examination

. /L.

V T m A x

. A , G. O
mm m ( ×) , j m

. A m ( ×) mm ( ×). E m
m m m . O m ( . ).

k m m - Low Power (10×)
m . I -

m m . O T x m
m m m. A m ,

m , m , m . m q , ,
Sources o Error: . I m

A . m m ,
T m , m , . T

, , - m RBC
m . A x

. C m m - ( . ). L
q m x q . I m , m mm m m ,

m . I m , m , m . C m
m : : . m m .

I m m ED A- High Power 40×
m ,

m . Sm A m m × j
m . I ( ×), m,

m x m ,
.

Red Blood Cell Distribution
TABLE 10.5
PERIPHERAL BLOOD FILM EVALUA ION Disorders

Terminology Description
P m -

, m , rouleaux Overlapping red blood cells, often referred

m C . to as a “stack of coins” caused by abnor-

T m mal or increased plasma proteins. May be

- m . D an artifact, if anticoagulated blood speci-

k . U m - men is allowed to stand for hours or if the

x m , m RBC thick part of blood smear is examined.

k k ; WBC x agglutination Clumps of red blood cells looking like

k . G bunches of grapes. This condition can

m. N falsely decreased the automated, calcu-

. T lated hematocrit and falsely increase the

, k - MCH and MCHC.

, . B k .