Page 238 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

222 PART 3 ■ Hematology Laboratory Assessments

TABLE 10.7 A Comparison of Normal Leukocytes in Peripheral Blood

Segmented

Neutrophil Band Neutrophil Lymphocyte Monocyte Eosinophil Basophil

Nuclear shape Lobulated Curved Round Indented or twisted Lobulated Lobulated

Chromatin Very clumped Moderately clumped Smooth Lacy Very clumped Very clumped

Cytoplasmic Pink Blue, pink Light blue Gray-blue Granulated Granulated

color

Granules Many Many Few or absent Many Many Many

Color of granules Pink, a few blue Pink Red Dusty blue Orange Dark blue

Average 56% 3% 34% 4% 2.7% 0.3%

percentage

Reporting Results m

R , , m ED A

m . V m .

S k . , x
. C

, , -

Procedure Notes m , S m m

■ T m q m m ,
C mm m (B x . ).

m . I m

WBC x m , ■ A m m m k m -

( C ) k , k

k . × /L.

■ T k m - ■ T , m , m
-q . T m , m.

k , L % k

m m . C m x m -

m . I m , . I

BOX 10.3

Technical Issues: Total Cell Counts and Corrective Action

Cell Type False Impact on Total Cell Count Resolution

>5 Nucleated RBCs/100 Elevated WBC Calculate WBC correction, if not automatically cor-

WBCs rected by blood cell counting instrument.

Agglutination of RBCs Decreased RBC count Warm blood specimen to 37°C for 15 minutes and then

retest.

Rouleaux None No correction available.

Platelet clumps or plate- Decreased platelet count If collected in EDTA anticoagulant, redraw blood speci-

let satellitism men in citrate anticoagulant and multiply the platelet

count by a factor of 1.1 to correct for dilutional effect of

liquid citrate anticoagulant.

Alternate strategy: Obtain a capillary blood specimen

and prepare blood smear at the patient’s point of care.