Page 173 - Clinical Hematology Atlas

CHAPTER 15 ACUTE MYELOID LEUKEMIA 157

MORPHOLOGY
Peripheral Blood: Myeloblasts, promyelocytes, and other immature myeloid precursors
Monoblasts and promonocyte and monocytes
6 Auer rods (see Figure 15-2, B)
Bone Marrow: Monoblasts large with abundant, moderate basophilic cytoplasm; some with
folded nuclei, one or more prominent nucleoli
Promonocytes, irregular, convoluted nucleus, cytoplasm slightly basophilic; granules;
occasional vacuoles
Eosinophils increased and dysplastic with many large granules, some basophilic
6 Auer rods
CYTOCHEMISTRY
Myeloperoxidase: positive (see Figure 15-2, E)
Nonspecific Esterase: positive (see Figure 15-6, E)
Specific Esterase: weakly positive in abnormal eosinophils

GENETICS
Recurrent genetic abnormality: inv(16) (p13.1q22) or t(16;16)(p13.1;q22); CBFB-MYH11
NOTE: These genetic abnormalities are diagnostic of AML and do not require a $20% blast
count.
IMMUNOPHENOTYPE
CD34 , CD117 , CD13 , CD33 , CD15 , CD4 , CD11b , CD11c , CD14 , CD64 ,
1
1
1
1
1
1
1
1
1
1
CD36 , CD65 1
1