Page 177 - Clinical Hematology Atlas

CHAPTER 15 ACUTE MYELOID LEUKEMIA 161

MORPHOLOGY
Peripheral Blood: Myeloblasts, 6 Auer rods

Oval macrocytes, microcytes, dimorphic red blood cell population, 6 basophilic stippling,
dysplastic nucleated red blood cells, multiple nuclei 6 abnormal nuclear shapes (see
Figure 18-1 A-D)
Bone Marrow: $20% of nonerythroid cells are myeloblasts, 6 Auer rods
$50% of all nucleated cells are erythroid precursors
Dysplastic erythroid precursors with megaloblastoid nuclei, round nuclei, fine chromatin,
multiple nucleoli, nuclear bridging
Cytoplasm deeply basophilic, often contain vacuoles that may fuse together (see
dyserythropoiesis, Figure 18-1 E-H)
Neutrophils: 6 dysplastic changes
Megakaryocytes: 6 dysplastic changes
CYTOCHEMISTRY
Myeloperoxidase: positive (see Figure 15-2, E)
Sudan Black B: positive (see Figure 15-2, F)
Periodic Acid–Schiff: block positivity in erythroblasts (see Figure 15-9, D)
Iron Stain: 6 Ringed sideroblasts (see Figure 18-1 I )

GENETICS
Recurrent genetic abnormalities: not defined
IMMUNOPHENOTYPE
Hemoglobin 1, glycophorin 1, CD13 , CD33 , CD117 6
1
1
FAB M6b
Pure Erythroid Leukemia

MORPHOLOGY
Peripheral Blood: Dysplastic nucleated red blood cells, multiple nuclei 6 abnormal nuclear
shapes
Oval macrocytes, microcytes, dimorphic red blood cell population, 6 basophilic stippling
Bone Marrow: $80% erythroid precursors without evidence of a myeloid component
Immature erythroid cells with deeply basophilic cytoplasm, round nuclei, one or more
nucleoli, vacuoles (some coalesced)
CYTOCHEMISTRY
Myeloperoxidase: negative
Sudan Black B: negative
Nonspecific Esterase: positive or negative
Periodic Acid–Schiff: block positivity in erythroblasts (see Figure 15-9, D)
Iron Stain: 6 Ringed sideroblasts (see Figure 18-1 I )
GENETICS
Recurrent genetic abnormalities: not defined
IMMUNOPHENOTYPE
CD71 , glycophorin 1, hemoglobin 1, CD13 , CD 33 , CD117 6
2
1
2