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Ethanol production from cassava pulp under fed-batch simultaneous

saccharification and fermentation at elevated temperature

Siriporn Lunprom*, Siriwan Khanpanuek, Apilak Salakkam

Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand, 40002
*Corresponding author: sirilun@kku.ac.th
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Abstract

Cassava pulp (CP) is considered one of the promising ethanol feedstocks, owing to its
ready and low-cost availability and its high carbohydrate but low lignin content. In the present

study, CP was used in a simultaneous saccharification and fermentation (SSF) at elevated
temperature (42 °C) to produce ethanol using a microbial isolate RTYK. Flask-scale experiment

showed that RTYK grew well in a wide range of temperature from 30 to 42 °C without significant
drop in its growth. The use of this isolate in a SSF process with CP concentration of 150 g/L
gave an ethanol production of 45.4 g/L, with a yield on CP and productivity of 0.30 g/g-CP, and

0.76 g/(L·h), respectively. Based on the carbohydrate content of CP, the fermentation

efficiency attained in this experiment was 78.4%. Further study will focus on SSF in a bioreactor
to gather substrate consumption and product formation data for designing a fed-batch SSF
process, which aims to increase ethanol yield and productivity. Based on the fermentation

results obtained in the fed-batch SSF, a mass balance will be calculated to assess the
feasibility of the process.

Keywords: cassava pulp, ethanol, process development, waste utilization, bioeconomy