Page 45 - Drug Discovery and Development: Prospects and Challenges

Drug Discovery and Development: Prospects and Challenges / 35

Figure 25: HPLC chromatogram of (A) asiaticoside standard,
Figure 25: HPLC chromatogram of (A) asiaticoside standard, (B) C. asiatica sample fraction using
(B) C. asiatica sample fraction using Activated Charcoal (CA FAC),
Activated Charcoal (CA FAC), (C) Ethyl Acetate:Methanol Fraction (SGEM FAC), and (D)
(C) Ethyl Acetate:Methanol Fraction (SGEM FAC),
Methanol Fraction (SGM FAC)
and (D) Methanol Fraction (SGM FAC)

Brothers and sisters,
Brothers and sisters,
Ladies and Gentlemen,
Ladies and Gentlemen,

Upscale Production of Pegaga Hydrogel
Upscale Production of Pegaga Hydrogel
The CA FAC was selected for the upscale hydrogel production based on the TLC and HPLC
The CA FAC was selected for the upscale hydrogel production based
results. The hydrogel films were prepared under three different concentrations comprising 0.25%,
on the TLC and HPLC results. The hydrogel films were prepared under
0.5%, and 1.0% sample fraction using the freeze-thaw technique with the combination of PVA and
three different concentrations comprising 0.25%, 0.5%, and 1.0%
PEG as the basis of the formulation. After undergoing five consecutive cycles of 16 hours freezing at
sample fraction using the freeze-thaw technique with the combination
-80 °C and 8 hours of thawing at room temperature, the hydrogels exhibited a non-sticky and opaque
of PVA and PEG as the basis of the formulation. After undergoing five
appearance (Figure 26).
consecutive cycles of 16 hours freezing at -80 °C and 8 hours of thawing

at room temperature, the hydrogels exhibited a non-sticky and opaque

appearance (Figure 26).
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