Page 24 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

CY17202 BIOORGANIC CHEMISTRY L T P C 3 0 0 3

OBJECTIVES:

 To understand bonding and stereo chemistry of organic molecules.
 To study the mechanism of addition and substitution reactions.
 To understand the kinetics of catalyzed bioorganic reactions.
 To study the functions of coenzymes and proton transfer reaction
 To understand the synthesis of biomolecules.

UNIT I BONDING AND STEREOCHEMISTRY 9
Atoms, electrons and orbitals - covalent bonds - octet rule - polar covalantbonds - electronegativity-
formal charge - resonance -acids and bases - Arrhenius and Bronsted Lowry theories - acid base
3
equilibria - sp hybridization - conformation analysis - ethane, butane and cyclohexane –cis- trans
isomerism. Stereochemicalactivity around the tetrahedral carbon – optical activity

UNIT II MECHANISMS OF SUBSTITUTION AND ADDITION REACTIONS 9
SN1 and SN2 reactions on tetrahedral carbon- nucleophiles- mechanism and steric effects –
nucleophilicaddition on acetals and ketals -aldehyde and ketone groups – reactions of carbonyl
group with amines- acid catalyzed ester hydrolysis – saponification of an ester- hydrolysis of
amides-ester enolates – claisen condensation – michael condensation.

UNIT III REACTIONS AND CATALYSIS 9
Kinetics and Energetics of reactions – transition state theory and arrhenius equation – reactivity and
catalysis
– intra molecular reactions – metal ion catalysis - carbonic anhydrase and carboxypeptidase A –
Covalent catalysis – Catalysis by organized aggregates and phases.

UNIT IV COENZYMES 9
Coenzymes (NAD + , FAD, PLP, CoA, Cobalamine, THF, Biotin, Lipoic acid) – acyl group
transfer – C-C bond formation and fission – catalysis of proton transfer reactions – transfer of
hydride ion – alkyl group transfer.

UNIT V BIOORGANIC REACTIONS 9
Microscopic reversibility – kinetic and thermodynamic control – thermodynamics of coupled
reactions - timing of bond formation and fission – terpenebiosynthesis – merrifield solid state
peptide synthesis - conformation of the peptide bond – sanger method for peptide and
DNAsequencing

TOTAL: 45 PERIODS
COURSE OUTCOMES:

On completion of the course students will be able to:
 Get familiarized with the bonding and stereo chemistry of bioorganic molecule.
 Distinguish between substitution and addition reactions of various functionalities.
 Describe the kinetics and energetics of catalyzed organic reactions.
 Apply the role of Coenzymes in bioorganic molecules.
 Identify synthetic strategies for peptides.

TEXT BOOKS
1. Carey, Francis A.‖Organic Chemistry‖.VII Edition, Tata MCGraw Hill, 2009
2. Page, M.I. and Andrew Williams ―Organic and Bio-organic Mechanisms‖. Pearson, 2010.

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 24