Page 29 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

BT17211 BIOCHEMISTRY LABORATORY L T P C

0 0 4 2

OBJECTIVES
 To learn and understand the principles behind the qualitative and
quantitative estimation of biomolecules
 To perform proteins, carbohydrates, lipids and metabolites estimation
 To perform enzyme assays.

EXPERIMENTS
1. General guidelines for working in biochemistry lab (theory)
2. Units of volume, weight, density and concentration measurements and their range in
biological measurements. Demonstration of proper use of volume and weight measurement
devices.
3. Accuracy, precision, sensitivity and specificity (theory)
4. Preparation of buffer –titration of a weak acid and a weak base.
5. Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars
and keto from aldo sugars.
6. Quantitative method for amino acid estimation using ninhydrin – distinguishing amino
from imino acid.
7. Protein estimation by Biuret and Lowry‘s methods.
8. Protein estimation by Bradford and spectroscopic methods.
9. Extraction of lipids and analysis by TLC.
10. Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
11. Enzymatic assay: phosphatase from potato.Estimation of glucose by GOD-POD method
after hydrolysis of starch with acid and specificity of the enzymatic method.

TOTAL HOURS: 60 HOURS

COURSE OUTCOMES:
Upon completion of the laboratory sessions, the students will be able to
1. Understand the basic principles of biochemical estimations and assays
2. Obtain practical knowledge in analysing various biomolecules both
quantitatively and qualitatively
3. Gain knowledge in preparation of biomolecules
4. Aquire expertise in operation of analytical techniques
5. Learn various methods of enzyme assays needed for clinical research.

REFERENCES:
nd
1. J.Jayaraman, Laboratory Manual in Biochemistry, 2 Edition, New Age
International Private Limited, January 2011.
2. S. K. Sawhney, Randhir Singh Eds, Introductory Practical Biochemistry, 5th or later
edition, Narosa Publishing House, New Delhi, 2014.

MA 17351 TRANSFORMS AND PARTIAL DIFFERENTIAL EQUATIONS L T P C
Common to AERO, AUTO, BT, CIVIL, EEE, MECH, MCT&FT 3 2 0 4

OBJECTIVES
 To introduce Fourier series which is central to many applications in engineering apart from its use in
solving boundary value problems.
 To acquaint the student with Fourier transform techniques used in wide variety of situations.
 To introduce the effective mathematical tools for the solutions of partial differential equations that
model several physical processes and to develop Z transform techniques for discrete time systems.

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 29