Page 123 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

Selection GuideⅡ

(Genomic DNA Prep Kit)

* The color of the solution in the process is for better understanding only.
Magnetic Bead Type Easy Type Solution Type Column Type

Cell lysis Cell lysis Cell Lysis & Precipitation Samples
Cell lysis
Cell lysis Cell lysis Cell lysis Cell Lysis & Precipitation Cell Lysis & Precipitation Samples
Samples
Cell line Bacteria Plasmid
Cell lysis Cell lysis Cell Lysis & Precipitation Samples Cell line Bacteria Plasmid
Cell line Bacteria Plasmid
Lysis
Protein Precipitation Magnetic Bead Biniding Cell line Bacteria Plasmid Lysis Lysis
Protein Precipitation Protein Precipitation Magnetic Bead Biniding Magnetic Bead Biniding
Lysis
Boiling 10min Protein Precipitation Magnetic Bead Biniding
75℃ 15min(act.) Boiling 10min
95℃ 5min(inact.) Boiling 10min
Column Binding Magnetic Bead Magnetic 75℃ 15min(act.) 75℃ 15min(act.)
Washing & Dry Bead 95℃ 5min(inact.)
DNA Precipitation Boiling 10min Column Binding 95℃ 5min(inact.) Magnetic Bead Magnetic Bead Magnetic
Column Binding
Magnetic
Bead
75℃ 15min(act.)
DNA Precipitation DNA Precipitation Washing & Dry Washing & Dry Bead
95℃ 5min(inact.)
Column Binding Magnetic Bead Magnetic
Preparation DNA Precipitation Washing & Dry Bead
Magnet
step Magnet Magnet
Binding and Washing
Washing DNA Elution
Washing Magnet Binding and Washing Binding and Washing
Washing Washing DNA Elution DNA Elution
Washing Washing
Binding and Washing
Magnet
Washing DNA Elution Magnet
Washing Magnet
DNA / RNA elution Magnet
DNA Elution DNA Elution
DNA / RNA elution DNA / RNA elution
Magnet
DNA Elution
DNA Elution DNA Elution DNA Elution Magnet
DNA / RNA elution
Magnet
Magnet
Eluted DNA DNA Elution DNA Elution Magnet Magnet Magnet
Eluted DNA Eluted DNA
Magnet
Eluted DNA
- Salting-out method
- Scale-up using a single kit - Quick extraction in large quantities
- Efficient extraction from small - Efficient extraction from
- Convenient extraction of gDNA of samples
Features quantity of sample various samples
from large quantities of samples - Simple and quick extraction process
- High-concentration gDNA - High-quality gDNA extraction
- High-concentration gDNA extraction - Difficulty in long-term storage
extraction
Extraction 30 mins. – 1 hour 30 min. 1 hour and 30 mins. 30 – 45 mins.
time
Dry
Magnetic Bead Cell Lysis Magnetic Bead Washing Magnetic Bead Elution
Binding
Type
Easy Type Add Bu er Boiling
DNA
Protein
Solution Type Cell Lysis Precipitation Precipitation Washing Elution
Cell Lysis Column Washing Dry Elution
Column Type Binding membrane
123
BIOFACT CATALOGUE