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3. Tracheal aspirate/ETT Aspirate

i. Aspirate the fluid through tracheostomy tube or ETT
ii. Place aspirated fluid into sterile container
HTAA, KUANTAN 4. Bronchial washing/BAL/Lung Tissue (Biopsy)
iii. Send to laboratory immediately.

i. Place aspirated fluid or tissue acquired from bronchoscopy into sterile container.
ii. Send to laboratory immediately.

2.9 Stool

1. Fresh stool

i. Collect faeces into a sterile container and send to the laboratory immediately.
ii. If the faeces is liquid, the container may be filled to one-third full (excessive
amount will result in spillage when opened).
iii. Enrichment medium i.e., Alkaline Peptone Water for Vibrios and Selenite F
for Salmonella and Shigella can be obtained from the laboratory for bedside
inoculation.

2. Rectal swab

i. Insert a cotton swab into rectum (1 inch beyond anal sphinter) and rotate once.
ii. Place the swab in Cary-Blair transport medium for isolation of enteric pathogen.

Notes :
1. Rectal swab is inferior substitute of fresh stool. It is to be taken if not possible
to obtain faeces.
2. For stool clearance culture in cases of typhoid and cholera, stool should be
sent upon completion of therapy.

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