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1.7 Cerebrospinal fluid

CSF specimens (1-2 ml) should be collected in a sterile container for microscopy and
culture.

1.8 Pus MICROBIOLOGY
1. Pus from undrained subcutaneous abscesses or sinus tract should be aspirated
with a sterile needle and syringe into sterile container.
2. In grains are visible in the pus as in mycetoma, these must be collected.

Note:
In mycetoma, if the crusts at the opening of the sinus tracts are lifted, grains can
often be found in the pus underneath.

1.9 Tissue
1. If possible, material should be obtained from both the middle and edge of the
lesions.
2. Small cutaneous, subcutaneous or mucosal lesions can often be excised
completely.
3. Tissue specimens should be placed in a sterile container without formalin.

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