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C) Catheter urine

HTAA, KUANTAN • Clean the sampling port with alcohol swab.
• Clamp the catheter tubing 4-5 cm below the sampling port until sufficient urine
collects.

• Insert needle attached to a syringe into the port using aseptic non touch
technique.
• Aspirate the required amount of uirne and place it in a sterile container.
• Send to the laboratory immediately.

Note:
• Do not collect the urine form the urine bag.
• Do not send urinary catheter tips for culture because tips are invariably
contaminated with urethral organisms.

2.11 Autopsy material

A. Blood

i. Aspirate 10 ml of the right heart blood either through skin and chest wall or
(through unopened heart) from right ventricle after removal of sternum into a
set of blood culture bottle.
ii. Avoid contamination with bacteria from the water faucet and with the enteric
bacteria.

B. Tissue

i. Best collected at an earlier stage before the body is being handled. Decontaminate
the skin or surface of heart or other organ before inserting or cutting out tissue
block.
ii. Collect the tissue and place in sterile container. Large piece is preferred and
accepted because aseptic collection is difficult.
iii. In the laboratory, tissue from suspicious area will cut aseptically and proceed
with culture.

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