Page 219 - pathology_services_handbook_5th_edition_2018

xiii. Using the corner of the spreader, make the blood film by joining the dots of MICROBIOLOGY
blood and spread them to make an even, thick film. Never stir the blood. Use
about 3-6 quick strokes. The circular thick film should be about 1 cm in diameter.
xiv. Label the slide. Let the smears dry on an even, flat surface before inserting into
slide jacket to be sent to the laboratory. Do not leave the slide to dry at high
ambient temperature and humidity as it will cause autofixing of the thick slide.
xv. Dispose spreader into sharp bin.

B. Syringe method

i. Follow standard procedure to collect blood, volume required about 1ml.
ii. Carefully remove needle, dispose safely into sharp bin.
iii. Follow the fingerprick method in preparing the thick and thin film.
iv. Dispose spreader & syringe into sharp bin.

4.2 Blood film for microfilaria

i. Collect a big drop of blood by pricking a finger or heel (baby).
ii. Make an oval thick blood film on a clean glass slide (3 x 2 cm).
iii. Dry it in a horizontal position, avoid it from dust or pests.
iv. Send immediately to the laboratory.

Note:
• Microfilaria blood samples must be taken between 10 pm and 2 am as this is
when nocturnal filaria goes into the blood stream.
• For adults, blood should be obtained from the finger while infants from the
heels. Venous blood is acceptable.
• Time specimen is taken must be noted on the request form to avoid rejection.

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