Page 32 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Development of Bacterial Nano Cellulose Wound Dressing Sheet Coated
with Peka Leaf Extract for Prevention and Treatment of Infected Wounds

Pichsinee Rattanaphan , Phetlada Rungruang
1
1
Advisors: Kuntida Khansuk , Pornvichai Temboot
1
2

Princess Chulabhorn Science High School Nakhon Si Thammarat
1
Thaksin University
2

Abstract
The objective of this study was developed and evaluated the bacterial nano cellulose wound dressing
sheet coated with Peka leaf extract for prevention and treatment infected wounds. The effect of different
carbon sources, including old coconut water, young coconut water, marinade-citrus fruits, marinade-
sweet fruit and water from Nypa palm, on forming of bacterial nano cellulose were also determined.
Then, the physical properties, strength, and water absorption property, of bacterial nano cellulose sheets
were tested. We founded that the bacterial nano cellulose cultured from marinade-citrus fruit exhibited
the highest tear resistance at 14.89 newtons per 30 square centimeters. Furthermore, the bacterial nano
cellulose cultured in from marinade-citrus fruit showed the highest water absorption property with an
average of 60.59 percent. Then, we selected the bacterial nano cellulose cultured from marinade-citrus
fruit mixed with gelatin to develop the wound dressing sheets coated with Peka leaf extract and test
their antimicrobial activity to Staphylococcus aureus by disc diffusion technique. We founded that the
concentration at 50% (w/v) of Peka leaf extract coated on this sheet showed an average inhibition zone
of 12.22 ± 0.22 mm.

Keywords: Bacterial nano cellulose wound dressing sheet, Peka leaf extract, Antimicrobial activity

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