Page 36 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

A Comparative study of the effectiveness of crude extracts from fungus
Beauveria bassiana Wild type strain, Overexpression pks14 FH strain,
Overexpression pks14 F30 strain as an inhibitor of amoeba cells

Dalad Rattanakrn , Chanakarn Chalearmmeung
1
1
Advisors: Bussarin Jitseng , Alongkorn Amnuaykanjanasin
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2

Princess Chulabhorn Science High School Satun
1
National Science and Technology Development Agency
2

Abstract
The objective of this research was to compare the effectiveness of crude extract metabolites from
Beauveria bassiana fungus Wild type strain, Overexpression pks14 FH strain and Overexpression pks14
F30 strain as an inhibitor of amoeba cells. There were 2 experiments: 1) Culturing and extracting 3
strains of Beauveria bassiana by using a simple extraction and use Ethyl acetate as a solvent, 2)
Compare the effectiveness of crude extract as an inhibitor of amoeba cells. We divided the experiments
into five groups. In each group using 1 mL of amoeba cells mixed with crude extract from 3 strain of
Beauveria bassiana, 100 microliters for 1 strain. The experimental control group are the tube 1 and 2.
There are tube combination of amoeba cells, Methanol and PBS, respectively. The result revealed that
the mortality rate of amoeba cells in tube 4 and 5 with a mixture of amoeba cells, crude extract
(Overexpression pks14 F30) and crude extract (Overexpression pks14 FH) is 100%. Followed by the
third tube is the combination of amoeba cells and crude extract (Wild type) which has the mortality rate
of amoeba cells is 28.2%. We can conclude that crude extract metabolites from Overexpression pks14
FH and Overexpression pks14 F30 of Beauveria bassiana are more effective as an inhibitor amoeba
cells than crude extract metabolites from Wild type of Beauveria bassiana.

Keywords: Beauveria bassiana, Wild type strain, Overexpression pks14 FH strain, Overexpression
pks14 F30 strain

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