Page 35 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Biofilm from Galangal to Protect Aspergillus flavus in Chopsticks

PaphichayaWarapho , Pattawan Ruangdech , Nichada Boonsilp

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Advisors: Benyaporn Patjareon , Orathai Lumsan , Prissana Wiriyajitsomboon
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Princess Chulabhorn Science High School Lopburi
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Kasetsart University
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Abstract
Chopsticks are popular in use, especially for food in Asia. There are many types of chopsticks such as
metal chopsticks and bamboo chopsticks. Bamboo chopsticks are most used because they are cheaper
than the others. However, the survey found that these types of chopsticks tend to have fungus such as
Aspergillus flavus on them because of holes or flaws in the packages. Due to the excessive use of plastic
packages become one of the causes of global warming that affect both life and environment. Therefore,
we should manufacture biological packages instead of plastic packages for the reason that they can
inhibit this type of fungus and protect consumers and the environment from harm. There are 2 methods,
method 1 is galangal extract inhibition test and method 2 is forming biofilm package. There are 2 results,
result 1 is galangal extract at 100,000 ppm has the best result to inhibit Aspergillus flavus and result 2
is biofilm with galangal extract at 100,000 ppm are gauzy, thin, tough and slightly flexible. Our study
demonstrated that the galangal extract could inhibit this fungus. These results would be an initial step
to further study the impact of using the galangal extract on manufacturing sector.

Keywords: galangal, Aspergillus flavus, fungus, biofilm, chopsticks

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