Page 41 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Preparation and Study of Efficacy of Beads Mixed with Fang Herbal
Extract for Medical Application

Benjapohn Khamthapthim , Phithaiporn Saesue
1
1
Advisors: Natpassorn Laonet , Krit Sutjarittangtham ,
1
2
Wipharat Chuachuad Chaiyasith , Worawut Kriangkrai
3
3

Princess Chulabhorn Science High School Phitsanulok
1
Pibulsongkram Rajabhat University
2
Naresuan University
3

Abstract
The objective of the present project was to study the suitable type and amount of polymer mixed with
herbal extract to forming beads that can inhibit the growth of bacteria. First, the effect of bacteria
inhibition of Fang herbal extract was studied. It was found that the extract can inhibit bacteria and had
a MIC value of 3.125 mg/ml. Second, the ratio of soluble polymer and Fang herbal extract in bead
forming for bacteria inhibition of Staphylococcus aureus was investigated. The results showed that the
efficacy of beads with different polymer ratios gave different abilities to store herbal extract. The beads
containing a polymer at a ratio of 40 A: 60 W have the most effective storage of extracts. As for the
study of emulsion stability, it was found that polymer at a ratio of 80 A:20 W and 100 A:0 W gave the
separation of layers. From the study of the properties of the beads at the ratio 40 A: 60 W using Fang
extract at the concentration equal to MIC and for the retention of extract study, it was found that the
amount of Brazilin 0.00189 mg/ml by bead during 0-120 minutes will gradually release but for the 120-
150 minutes, there are large amounts of extracts released. From the study of bead swelling, it was found
that the beads had small swelling in the period of 0 - 15 minutes and there was no further swelling. For
a study of a physical characteristics, it was observed that the beads were spherical in shape, smooth
surface, with an average diameter of 1.75 mm. When the inhibitory activity was tested, it was indicated
that the bead mixed with herbal extract at the concentration equal 6MIC gave the most effective
inhibition of infection with the diameter of the average inhibitory area of 2.18 cm. Finally, to develop
an initial product containing beads from polymer encapsulated herbal Fang extracts for medical uses,
when cream was mixed, the suitable formula ratio of cream: bead was 5:1 with the diameter of the
average inhibitory zone of 2.09 mm. It was also found that when shaking the cream, it will increase the
releasing efficiency of the extract from beads.

Keywords: Fang herbal extract, beads, Polymer, Cream mixed with beads

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