Page 42 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Study the efficacy of Myrtaceae family’s extraction on the Streptococcus
mutans inhibition for teeth decay prevention and Study of adhesion and

detachment of extracts compare with carbonated water
and anticaries agents at the enamel layer of crown and root
for the enamel coating development

Kittinut Banchajarurat , Tanapat Buranaprasertsuk
1
1
Advisors: Pornpan Chomwong , Warangkhana Thuphakde ,
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1
Pagakrong wanapaisa , Rangsima Sakoolnamarka
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2

Princess Chulabhorn Science High School Pathumthani
1
Mahidol university
2
Chulalongkorn University
3

Abstract
In this study, the efficacy of enamel from Myrtaceae family’s: apple, guava and jambul, compared with
chlorhexidine. To extract the crude extract in each plant, the extract was obtained for oral antibacterial
testing by 95% ethanol extraction, filtered and dried under vacuum condition. And test for inhibition of
Streptococcus mutans infection causing caries decayed tooth by disc diffusion on Mueller Hinton agar
at initial concentrations of 1: 2, 1:10, 1: 100 and 1: 1000 over 12, 24 and 48 hours, repeated. 6 times to
find the mean and standard error. It was found that at the initial concentration, chlorhexidine effective
in inhibiting Streptococcus mutans bacteria was the most followed by the guava from the antibacterial
study and rose apple respectively. From the study of inhibition on Streptococcus mutans was found that
Myrtaceae family crude extract had maximun ability to prohibit Streptococcus mutans at 12 h.
Chlorhexidine had the highest inhibitory efficacy at 48 hours and guava extract can inhibit bacteria. The
most common Streptococcus mutans was with 60% inhibition percentage, followed by rose apple with
50% inhibition percentage and jambul was unable to inhibit these bacteria. In part of the dental adhesion
and detachment experiment, the teeth were soaked in extracts from the Myrtaceae family, chlorhexidine,
fluoride and carbonated water coatings to test the adhesion of substances then the teeth that were soaked
in carbonated water were further soaked in various extracts. To test the detachment and adhesion of the
extract compared with chlorhexidine, then it was further developed into tooth enamel.

Keywords: Myrtaceae family, Decayed tooth, Streptococcus mutans, Chlorhexidine, Disc diffusion

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