Page 47 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

The Study of Antibacterial in Chili Pepper and Pepper Extract

Nongnapas Hengpatomwat , Panyawee Phuimart , Suphitcha Komonsai
1
1
1
Advisors: Jansuda Saengsoda , Jesada Karnchanajindanun , Natenapha Kledchine
2
1
1

Loeipittayakom School
1
Loei Rajabhat University
2

Abstract
The project's purpose is to study and to compare the extracted of pepper and chili pepper. The bacteria
that were used are Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Enterobacter
aerogenes and Pseudomonas aeruginosa. The extracts of chili peper, peper, bacteria on the nutrient
agar and 70% Ethanol were cultivated into peper disc. Incubate the mixture in the incubator to evaporate
the solvent. Put the mixture (chili pepper, pepper, 70% Ethanol) on top of each bacteria that was on top
of nutrient agar. Incubated for 18 - 24 hours, then measure the inhibition zone or clear zone of each
paper disc on each bacterium. The goals of methodology were as to see if the pepper extracted can
inhibit Escherichia coli (Clear zone: 1 cm) and Pseudomonas aeruginosa (Clear zone: 1 cm). The chili
pepper can inhibit only Pseudomonas aeruginosa (Clear zone: 0.8 cm) as same as the control disc.

Keywords: Extracted, chili pepper, pepper, Staphylococcus aureus, Escherichia coli, Klebsiella
pneumonia, Enterobacter aerogenes and Pseudomonas aeruginosa

35