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1152 Part IX: Lymphocytes and Plasma Cells Chapter 74: Lymphopoiesis 1153

Mouse
LT-HSC CD34 neg Flt3 neg
hi
hi
(Lin neg Sca Kit CD150+CD48–)

MPP CD34+Flt3 neg IL7R neg
hi
hi
(Lin neg Sca Kit )
CMP Myeloid Lymphoid
hi
CD34+Flt3 IL7R neg
LMPP neg
(Lin Kit+Sca+)
CD34+IL7R+
Io
Io
CLP (Lin neg Kit Sca thy1 neg )
MEP GMP

DC B NK T
Bone marrow Thymus
A
Human
HSC/MPP
HSC/MPP
CD34+Lin neg CD38 neg
LMPP BM “LMPP” neg
neg
neg
CD34+Lin CD38 CD10
CD45RA+CD62L hi
Myeloid UCB/BM “MLP”
Lymphoid CD34+Iin neg CD38 neg CD45RA+CD10+
CLP
CMP UCB “MLP”
neg
CD34+CD38 CD45RA+CD7+
BM CLP
CD34+Iin neg CD45RA+CD10+

MEP GMP

DC B NK T
Bone marrow Thymus
B
Figure 74–2. Postnatal pathways of lymphopoiesis in mice and humans. The key immunophenotype used to isolate each population is shown in
boxes on the right. In parentheses under the main immunophenotypes are other markers also associated with each population. The exact relation-
ship between lymphoid-primed multipotential progenitors (LMPPs) and common lymphoid progenitors (CLPs), and between LMPPs and myeloid
progenitors remains controversial, as does the main cell type that initiates thymopoiesis (all shown as dashed lines). In both mice and humans, den-
dritic cells (DCs) can be produced in vitro from all prospectively identified lymphoid progenitors as well as myeloid progenitors. A. Murine lymphoid
progenitor pathways. Populations with multilineage potential include long-term hematopoietic stem cells (LT-HSCs) and multipotential progenitors
(MPPs). FLT3+ LMPPs, have full lymphoid (T-, B-, and natural killer [NK] cell developmental potential) and limited myeloid (nonclonogenic, mostly
monocyte) potential. In the CLP, all myeloid potential is lost and full lymphoid potential remains. B. Human lymphoid progenitor pathways. Popula-
tions with multilineage potential include HSC and LMPP (aka multilymphoid progenitor [MLP]), which have full lymphoid (T-, B-, and NK cell develop-
mental potential) and limited myeloid (mostly monocyte) potential. The CD10+ CLP has predominantly B, and some NK and T potential and is almost
devoid of myeloid potential. Alternative phenotypic markers reported for each progenitor type with similar lineage potential are shown. BM, marrow;
GMP, granulocyte-macrophage progenitor; MEP, megakaryocyte-erythroid progenitor.

Kaushansky_chapter 74_p1149-p1158.indd 1152 9/18/15 2:26 PM