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PART THREE
Hematology Laboratory Assessments
10 Basic Laboratory Assessment o
Principles o Blood Collection
CHAPTER Erythrocytes, Leukocytes, and Platelets
KEY TERMS
anisocytosis mean corpuscular volume (MCV) polychrom asia
erythrocyte sedim entation rate (ESR) mean corpuscular hemoglobin (MCH) polychrom atophilia
hem oglobin mean corpuscular hemoglobin reticulocyte
leukocytosis concentration (MCHC) rouleaux
leukocytpenia poikilocytosis
LEARNING OUTCOMES
The Complete blood count Quantitative assessment of leukocytes
■ List components of a complete blood count (CBC). ■ Calculate a manual WBC cell count when given the dilution and
■ De ne abbreviations: RBC, WBC, Hgb, Hct, and retic. numbers of cells counted.
■ Name two conditions that can produce leukocytosis.
Quantitative assessment of erythrocytes
■ Name two conditions that can produce leukocytopenia.
■ Calculate a manual RBC cell count when given the dilution and num- ■ Calculate an absolute cell count.
bers of cells counted. ■ Compare some reasons for eosinophilia and basophilia.
■ Describe the principle of the cyanmethemoglobin assay for determi- Explain the method and clinical applications of the neutrophilic
nation of hemoglobin. hypersegmentation index.
■ Describe the measurement of a microhematocrit. ■ Correct a total white blood cell count when nucleated red blood
■ Compare RBC, hemoglobin, and hematocrit values using the rule cells are present.
of three.
■ De ne each of the erythrocyte indices: mean corpuscular volume Other leukocyte-related assessments
(MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular Critique the purpose and clinical applications of assessing neutro-
hemoglobin concentration (MCHC). philic function and the killing ability of granulocytes.
■ Apply the appropriate formulas and calculate the MCV, MCH, and ■ Name a classic application of the leukocyte alkaline phosphatase
MCHC when given the erythrocyte values. (LAP) test.
■ Classify RBC morphology based on erythrocyte indices. ■ Explain the purpose of the erythrocyte sedimentation rate (sed rate).
■ Compare the morphological appearances of reticulocytes stained ■ Discuss the classic history of the sed rate.
with Wright stain and a supravital stain, such as new methylene blue.
■ Give the normal value of the uncorrected reticulocyte count. Quantitative assessment of platelets
■ De ne the terms shift or stress reticulocytes. ■ Describe the appearance of a mature platelet in a platelet count
When given the necessary laboratory results, calculate the corrected preparation.
reticulocyte count, absolute reticulocyte, and reticulocyte produc- ■ Describe the manual calculation of a platelet count.
tion index and compare the values to normal reference ranges. Semiquantitative grading of erythrocytes
Explain the terms reticulocyte hemoglobin concentration, immature Describe the process of evaluating a peripheral blood lm, including selec-
reticulocyte fraction, and reticulated hemoglobin content ■
tion of the correct area and observations to be made at each magni cation.
Correlate polychromatophilia on a blood smear with reticulocyte De ne the terms anisocytosis and poikilocytosis
results. ■
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