Page 227 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

CHAPTER 10 ■ Basic Laboratory Assessment of Erythrocytes, Leukocytes, and Platelets 211

Hematocrit (Packed Cell Volume)

TABLE 10.1 Manual Total Cell Counts
T hematocrit Hct , packed cell volume, m -

m k RBC m-
Standard , m m q . T

Blood Microscope m m . A m -

Type of Specimen Objective

Cell Dilution Diluent Power m .

RBC 1:100 0.85% NaCl 40× W ,
m ,
WBC 1:20 3% acetic acid, 10× . T m

1% ammonium RBC m . I x

oxalate, 1% hydro- SI m.

chloric acid
A m m m -
Platelet 1:100 1% ammonium 40× m m . T m

oxalate phase microscopy m m (MCV)

m

RBC m m m . H m

m m

QUAN I A IVE ASSESSMEN OF m .

ERY HROCY ES S m m m m -

m m .
Hemoglobin Measurement in the Laboratory S m

q ED A
T m hemoglobin Hb m

CBC. A m m - RBC k .

m m m , m .

m m m . A m - P m m

m m q k m m .

m k m .

T m m , H C, m - Rule of Three

m m m D k ’ A q k k m m m

m ; m ; - “ .” T m m -

m (KH PO ), m m

m m ; m (H × = HC ± %).
m m z RBC . W Example: I m . / L

m m m x - m m %, m

m , m H CN m m - m m . T

q m . ( % %).

H m m m - T m m , m

m m m . A m -

m . T m - m m m m .

D k ’ , m m.

S m m m Blood Volume Measurement

m m . H m

m ( m ), x m I m , m

WBC , m - H

C m S. m m

S m- mm m ,

. I m m - , .

, m m q m m , m m

. % , m x m , m . I m m m m .

x m WBC , P m m m m . A -

m m m m -

, m , E , I- m ,

, % m- , j m -

m . I m C S m j

, m : m . R

m . m m mm , m