Page 241 - Clinical Hematology Atlas

CHAPTER 24 BODY FLUIDS 225

A B
FIGURE 24–1A Wright-stained cytocentrifuge FIGURE 24–1B Wright-stained cytocentrifuge
slide demonstrating a concentrated button of cells slide from a cerebrospinal fluid containing few
within the marked circle. cells, demonstrating the importance of marking
the cell concentration area.

blood smears. This is because the larger, and usually more significant, cells are likely to be
pushed to the end of the slide.
When examining the cytospin slide, the entire cell button should be scanned under the
103 objective to search for the presence of tumor cells. The 503 or 1003 oil immersion lens
should be used to differentiate the white blood cells. For the performance of the differential,
any area of the cell button may be used, but if the cell count is low, a systematic pattern starting
at one end of the side of the button and working toward the other is recommended.
Any cell that is seen in the peripheral blood may be found in a body fluid in addition
to cells specific to that fluid (e.g., mesothelial cells, macrophages, tumor cells). However, the
cells look somewhat different than in peripheral blood, and some in vitro degeneration is
normal. The presence of organisms, such as yeast and bacteria, should also be noted (see
Figures 24-12 to 24-14).