Page 11 - Ranger SPM 2022 Biology

Biology SPM Chapter 1 Introduction to Biology and Laboratory Rules
2. There are substances that can and cannot be disposed into the sink in a biology
laboratory.

Substances that can be disposed into Substances that cannot be disposed
the sink: into the sink:
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• Substances with pH level of 5 to 9 • Organic solvent
• Liquids or solutions with low concentration • Substances with pH level of less than 5 or
and non-hazardous more than 9
• Chemical substances
• Solid waste
• Heavy metals
• Volatile substances
Laboratory • Organic waste
Rules • Toxic, reactive and radioactive substances
INFO
Form 4
3. Table 1.1 shows methods for managing biological wastes according to category.
Table 1.1 Methods for managing biological wastes according to category

Waste substances Example Method of handling the biological
category waste

Category A Sharp equipment that can Inserted into a container that is
(Sharp wastes) cause injury when touched specially prepared for sharp wastes
and does not need to be sterilised.

Category B Biological solid wastes Packed first into a biohazard
(Non-sharp wastes) plastic bag that is autoclave
resistant, sterilised in an autoclave
to decontaminate and put into a
biohazard bin.

Category C Carcass, organ, tissue Wrapped in absorbent material (such
(Animal carcasses) remains of laboratory as tissue paper), packed neatly into a
animal or test animals biohazard plastic bag and frozen.

Category D Broth culture, liquid All biological liquid wastes must be
(Liquids) medium decontaminated by autoclaving before
being disposed. Biological liquid waste
that has been sterilised must be
disposed immediately.

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F4 Chapter 1.indd 4 3/29/22 3:10 PM