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Sains Tingkatan 5 Bab 1

6. Ulangi langkah 4 dan 5 dengan meletakkan tapak tangan kiri yang dibasuh dengan air pada
permukaan agar nutrien steril di dalam piring Petri B, kemudian tapak tangan kiri yang
dibasuh dengan air dan sabun pada permukaan agar nutrien steril di dalam piring Petri C.
Repeat steps 4 and 5 by placing the left palm washed with water onto the surface of sterile nutrient agar in Petri
BAB
dish B, then the left palm washed with water and soap onto the surface of sterile nutrient agar in Petri dish C.
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7. Simpan kesemua piring Petri dalam keadaan telangkup di dalam inkubator pada suhu 37°C
selama tiga hari.
Keep all the Petri dishes in inverted condition in an incubator at 37°C for three days.
8. Rekodkan pemerhatian selepas hari ketiga.
Record the observations after three days. K2P4

Kaedah 1. Tiga piring petri steril disediakan dan dilabelkan sebagai A, B and C.
Method
Three sterile petri dishes were prepared and labelled as A, B and C.
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2. 10 cm agar-agar nutrien steril dituangkan ke dalam setiap piring Petri.
EKSPERIMEN WAJIB
10 cm sterile nutrient agar were poured into each Petri dish.
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3. Seorang murid dipilih bagi mewakili tiga jenis keadaan tangan, iaitu tangan yang tidak dibasuh,

tangan yang dibasuh dengan air sahaja dan tangan yang dibasuh dengan air dan sabun.
One student were chose to represent three conditions of hands which are unwashed hands, hands washed with
water only and hands washed with water and soap.
4. Tapak tangan kiri yang tidak dibasuh diletakkan pada permukaan agar-agar nutrien steril

di dalam piring Petri A selama 1 minit.
The unwashed left palm was placed onto the surface of sterile nutrient agar in Petri dish A for 1 minute.

5. Tangan dialihkan dan piring Petri A ditutup dengan penutupnya dan direkatkan dengan
serta-merta.
The hand was removed and the Petri dish A was covered with lid and sealed immediately.
6. Langkah 4 dan 5 diulangi dengan meletakkan tapak tangan kiri yang telah dibasuh dengan air

pada permukaan agar nutrien steril di dalam piring Petri B, kemudian tapak tangan kiri yang
telah dibasuh dengan air dan sabun pada permukaan agar nutrien steril di dalam piring Petri C.
Steps 4 and 5 were repeated by placing the left palm that has been washed with water onto the surface of sterile

nutrient agar in Petri dish B, then the left palm that has been washed with water and soap onto the surface of
sterile nutrient agar in Petri dish C.
7. Kesemua piring Petri disimpan dalam keadaan telangkup di dalam inkubator pada suhu

37°C selama tiga hari.
All the Petri dishes were kept in inverted condition in an incubator at 37°C for three days.
8. Pemerhatian direkodkan selepas hari ketiga.

Observations were recorded after 3 days. K1P5

Pemerhatian Piring Petri Keadaan tangan Bilangan koloni bakteria
Observation Petri dish Condition of hands Number of bacterial colonies

K3 A Tangan yang tidak dibasuh Sangat banyak
PI Unwashed hands Vast
1 Basuh tangan dengan air sahaja Many
2 B Washed hands with water only Banyak
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S C Basuh tangan dengan air dan sabun Sedikit
Washed hands with water and soap
A few
K3P1 K3P2 K3P3
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