Page 91 - APS(2022)_TG5 _Edisi Guru

Sains Tingkatan 5 Bab 1
Tarikh:

EKSPERIMEN Kesan Cahaya terhadap Pertumbuhan Mikroorganisma
Wajib 1.4 Effect of Light on the Growth of Microorganisms
Pendekatan inkuiri
BAB
PEKA
Video Eksperimen
1
GURU
Tujuan Mengkaji kesan cahaya terhadap pertumbuhan bakteria. GURU Kesan Cahaya terhadap
Aim To study the effect of light on the growth of bacteria. Pertumbuhan Mikroorganisma K1P1
Effect of Light on the Growth of
Microorganisms
Pernyataan Bagaimanakah cahaya mempengaruhi pertumbuhan bakteria?
Masalah How does light affect the growth of bacteria?
Problem Statement
Hipotesis Bakteria tumbuh pesat di dalam gelap berbanding dengan keadaan bercahaya .
Hypothesis
Bacteria grow rapidly in the dark compared to the bright condition. K1P2

Keamatan cahaya
EKSPERIMEN WAJIB
Pemboleh Ubah (a) Dimanipulasikan :
Variables
Manipulated Light intensity
K1
PI (b) Bergerak balas : Kekeruhan bubur nutrien
1 Responding Cloudiness of the nutrient broth
2
3 Kuantiti bakteria
(c) Dimalarkan :
4
5 Constant Quantity of bacteria K1P3
S

Bahan Kultur bakteria (Bacillus subtilis), bubur nutrien steril dan kapas steril.
Materials Bacteria culture (Bacillus subtilis), sterile nutrient broth and sterile cotton wool.

Radas Tabung uji steril, silinder penyukat steril dan picagari steril 1 cm³.
Apparatus Sterile test tubes, sterile measuring cylinders and sterile 1 cm³ syringe. K1P4

Prosedur A B C
Procedure
Kapas Kapas
Cotton wool Cotton wool
K2
PI
1 5 cm bubur nutrien
3
2 5 cm nutrient broth 5 cm bubur nutrien
3
3
3
3 + 5 cm nutrient broth
4 1 cm kultur bakteria 3 +
3
3
S 1 cm bacterial culture 1 cm kultur bakteria
1 cm bacterial culture
3
Di dalam almari bertutup Di atas meja di dalam makmal
Inside a closed cupboard On a table in the laboratory Di bawah cahaya terang lampu meja
Under the bright light of a table lamp
3
3
1. Masukkan 5 cm bubur nutrien steril dan 1 cm kultur bakteria ke dalam tiga tabung uji
steril berlabel A, B dan C seperti yang ditunjukkan dalam rajah.
Add 5 cm of sterile nutrient broth and 1 cm of bacterial culture into three sterile test tubes labelled A, B and C as
3
3
shown in the diagram.
2. Sumbat mulut setiap tabung uji dengan kapas steril.
Stuff the opening of each test tube with sterile cotton wool.
3. Letakkan tabung uji A ke dalam almari yang bertutup.
Place test tube A inside a closed cupboard.
4. Letakkan tabung uji B di atas meja di dalam makmal.
Place test tube B on the table in a laboratory.
5. Letakkan tabung uji C di bawah cahaya terang lampu meja.
Place test tube C under the bright light of a table lamp.
6. Perhatikan kekeruhan bubur nutrien selepas dua hari.
Observe the cloudiness of the nutrient broth after two days.

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01 BAB 1.indd 10 7/28/21 2:56 PM