Page 89 - APS(2022)_TG5 _Edisi Guru

Sains Tingkatan 5 Bab 1
Tarikh:
Kesan Nutrien dan Kelembapan terhadap

EKSPERIMEN 1.3 Pertumbuhan Mikroorganisma
Wajib Effects of Nutrients and Humidity on the Growth of Pendekatan inkuiri
Microorganisms
BAB
PEKA
1
Tujuan Mengkaji kesan nutrien dan kelembapan terhadap pertumbuhan mikroorganisma.
Aim To study the effects of nutrients and humidity on the growth of microorganisms. K1P1

Pernyataan Adakah nutrien dan kelembapan mempengaruhi pertumbuhan mikroorganisma?
Masalah Do nutrients and humidity affect the growth of microorganisms?
Problem Statement

Hipotesis Kehadiran nutrien dan kelembapan meningkatkan kadar pertumbuhan mikroorganisma.
Hypothesis
The presence of nutrients and humidity increases the growth rate of microorganisms. K1P2

Pemboleh Ubah (a) Dimanipulasikan : Kehadiran nutrien dan air
EKSPERIMEN WAJIB
Variables
Manipulated Presence of nutrients and water
(b) Bergerak balas : Bilangan koloni bakteria
Responding Number of bacterial colonies
(c) Dimalarkan : Kuantiti bakteria / Jenis bakteria
Constant The quantity of bacteria / The type of bacteria K1P3
Bahan Agar-agar nutrien steril, agar-agar steril tanpa nutrien dan kultur bakteria (Bacillus subtilis).
Materials Sterile nutrient agar, sterile agar without nutrient and bacterial culture (Bacillus subtilis).

Radas Piring Petri steril dengan penutup, picagari steril 1 cm³, silinder penyukat steril, pita selofan dan
Apparatus ketuhar.
Sterile Petri dishes with lids, sterile 1 cm³ syringe, sterile measuring cylinder, cellophane tape and oven. K1P4

Prosedur
Procedure Agar-agar Agar-agar Agar-agar
tanpa nutrien nutrien nutrien kering
Agar without Nutrient agar Dry nutrient agar
K2 nutrient + +
PI + Piring Petri A Piring Petri B Kultur bakteria Piring Petri C Kultur bakteria
Petri dish C
Petri dish A
Petri dish B
Kultur bakteria
1 Bacterial culture Bacterial culture Bacterial culture
2
3 1. Sediakan susunan radas seperti di atas.
4 Set up the apparatus as shown above.
S
3
2. Tuangkan 10 cm agar-agar tanpa nutrien steril, agar-agar nutrien steril, agar-agar nutrien
3
kering dan 1 cm kultur bakteria masing-masing ke dalam piring Petri A, B dan C.
Pour 10 cm of sterile agar without nutrient, sterile nutrient agar, dry agar nutrient and 1 cm of bacterial culture
3
3
into Petri dishes A, B and C respectively.
3. Tutup piring-piring Petri itu dengan penutup dan lekatkan dengan pita selofan.
Cover the Petri dishes with lids and seal the lids using cellophane tape.
4. Simpan kesemua piring Petri itu dalam keadaan telangkup pada suhu bilik.
Keep all the Petri dishes in inverted condition at room temperature.
5. Rekod pemerhatian selepas dua hari.
Record the observations after two days.
Kaedah 1. Susunan radas disediakan seperti yang ditunjukkan. / The apparatus was set up as shown.
Method
2. 10 cm agar-agar tanpa nutrien steril, agar-agar nutrien steril, agar-agar nutrien kering dan
3
3
1 cm kultur bakteria masing-masing dituangkan ke dalam piring Petri A, B dan C.
10 cm of sterile agar without nutrient, sterile nutrient agar, dry agar nutrient and 1 cm of bacterial culture were
3
3
poured into Petri dishes A, B and C respectively.
3. Kesemua piring Petri ditutup dengan penutup dan penutup dilekatkan dengan pita selofan.

All the Petri dishes were covered with lids and the lids were sealed using cellophane tape.

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