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          earth is  How do                                                            A steady beam of
          What on                                                                     Electron gun
                                                                                      high-energy electrons is
                                                                                      created either by thermal
                               electron                                               electrical fields
          that?                                                                       fired into the machine,
                                                                                      energy (thermionic guns) or
                                                                                      (field-emission guns) .
                               microscopes                                            Lenses
                                        •
                                                                                      A series of magnetic lenses
                                                                                      bend and focus the beam
                                                                                      into a precise spot to
          Guitar string        operate?                                               ensure only a specific part
          Magnification: x75                                                          of the sample is hit by the
          Here, you can see the inside of a                                           beam at any one time.
          'super-wound' guitar string under a
          scanning electron microscope.
                                We put these marvellous magnification
                                machines under the microscope

                                       hen the scanning   The core principle of an SEMis that it
                                       electron microscope   uses a 'tracing' technique to produce
                                W  ISEM) was unveiled in   a replicated 3D image of the original
                                1935, its reception was lukewarm at   sample being studied. It does this by
           Fly eye              best. Despite the potential to   scanning its electron beam over an
          Magnification: xlBO
          This is a scanning electron microscope   magnify objects up to 30o,ooo   object and measuring the electrons
           image of the drosophilidae compound   times, scientists struggled to see a   given off at a particular point. Using
          eye of a fruit fly.
                                commercial use for the bulky and   this process it can create a 'trace' of
                                                                                      Sweep
                                expensive machines. However,   the object, and output an amplified
                                                                                      Scanning electromagnetic
                                their application was vastly   image to a display. This is made   coils move the focused
                                underestimated, and today more   possible byscanningcoils, which   beam across the sample
                                                                                      in rows, so that the whole
                                than 50,000 are in use worldwide,   create a magnetic field that moves the   sample is subjected to
                                largely for industrial purposes.   beam across the surface of the   the beam.
                                SEMs have a variety of modern-day   sample. The smaller the area the
                                uses, from forensics to microchip   beam sweeps across the larger the
           Snowflake            production and insect observation.   magnification will be, and vice versa.
          Magnifzcation: xlOO    SEMs have many advantages over   One of the most important
           Computer-generated colours have been
           used on  this snowflake to highlight its   other methods of magnification   aspects of using an SEM is
           crystalline structure.   such as optical microscopes. For   preparing a sample for observation
                                example, they do not rely on light   and ensuring that there is nothing
                                for their images, which isa major   that could hinder the final image.
                                drawback of their optical   Samples must be thoroughly
                                counterparts. Light is unpredictable   cleaned to get rid of any dust, debris
                                as it can diffract and bend around   or alien material not native to the
                                objects, potentially making   sample that could skew results. The
                                observations very difficult. As   sample must also be able to conduct
          Moth                  their name suggests, scanning   electricity. If it can't, electrons will
          Magnification: x75    electron microscopes instead rely   not leave its surface when struck by
           In this close-up view of a pyralidae moth   on the release of electrons to   the beam. Objects that aren't
          you can see the side of its head, including
          its compound eye and its proboscis.   make observations.   already conductive will be coated in
                                 Inside a scanning electron   a fine layer of gold or platinum in a
                                microscope's casing are an electron   process known as 'sputter coating'.
                                gun, several coils, and condensing   This also prevents the sample
                                lenses that work together to observe a   becoming damaged by the beam
                                target sample in super-fine detail.   during observation.
                                                                                      Bad vibrations
                               '' SEMs have a variety of modem­                       The sample is  placed on a
                                                                                      stage inside a chamber of
           Peacock mite        day uses, from forensics to microchip                  the machine. This must be
          Magnification: x260                                                         kept extremely still as
           Here's a  pest commonly found in the                                       SEMs are very sensitive
          tropics known as a peacock mite,   production and insect observation ''     to vibrations.
          imaged here on a tea stem.

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