Page 42 - R2017 Final_BE Biomedical Curriculum and Syllabus - REC

Department of BME, REC

3. Trevor palmer and Philip L Bonner, “Enzymes: Biochemistry, Biotechnology, Clinical
nd
Chemistry”, 2 Edition, Woodhead Publishing, 2009.

REFERENCES:
1. Keith Wilson & John Walker, “Practical Biochemistry - Principles & Techniques”, Oxford
University Press, 2009.
2. Pamela.C.Champe & Richard.A.Harvey, “Lippincott Biochemistry Lippincott’s Illustrated
th
Reviews”, 6 Edition, LWW publishers,2013.

BM17311 BIOCHEMISTRY AND PHYSIOLOGY LABORATORY L T P C
0 0 4 2
OBJECTIVES
• Estimation and quantification of biomolecules.
• Separation of macromolecules.
• Estimation and interpretation of biochemical parameter.

LIST OF EXPERIMENTS:
1. General guidelines for working and functional component of biochemistry lab
2. Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions
3. Spectroscopy: Determination of absorption maxima (λmax) of a given solution
4. General tests for carbohydrates, proteins and lipids.
5. Preparation of serum and plasma from blood.
6. Estimation of blood glucose.
7. Estimation of creatinine.
8. Estimation of urea.
9. Estimation of cholesterol
10. Assay of SGOT/SGPT.
11. Separation of proteins by SDS electrophoresis(Demo)
12. Separation of amino acids by thin layer chromatography
13. Identification of Blood groups
14. Estimation of Hemoglobin
15. Total WBC Count
16. Estimation of ESR
17. PCV, MCH, MCV, MCHC
TOTAL: 60 PERIODS

OUTCOMES:
On completion of the course students will be able to
• Identify the Biochemistry laboratory functional components
• Comprehend the basics principle of preparation of buffers.
• Have a sound knowledge of qualitative test of different biomolecules.
• Explore the basic knowledge about Biochemical & haematological parameters and their
interpretation in Blood sample.
• Have a sound knowledge of separation technology of proteins and amino acids.

LAB REQUIREMENT FOR A BATCH OF 30 STUDENTS:
1. Colorimeter 2 Nos
2. Spectrophotometer 1 No.
3. pH meter 1 No
4. Weighing balance 1 No
5. Refrigerator 1 No

Curriculum and Syllabus | B.E Biomedical Engineering | R 2017 Page 42