Page 8 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

sterilization – Types of culture medium – Oxygen requirements of industrial fermentation – Mass
transfer in fermentation – Determination of KLa values – Factors affecting KLa values in fermentation.
UNIT-II INDUSTRIALFERMENTATIONPROCESSES 9
Aerobic and anaerobic fermentations – Development of inocula for industrial fermentation – Batch
culture, continuous culture, fed batch culture – Comparison of batch and continuous culture –
Submerged and solid state fermentation for the production of enzymes – Immobilization of enzymes –
Biocatalysis in organic media using enzymes – Biotransformation with crude enzymes and wholecells.
UNIT-III PRODUCTION OF ENZYMESANDMETABOLITES 9
Production of Proteases, Cellulases, Lipase, Amylase, Glucose isomerase, Pectinase, Peroxidase –
Production of organic acids (Citric acid, Lactic acid) – Production of antibiotics (Penicillin,
streptomycin) – Production of vitamins (Vitamin B12, Riboflavin) – production of amino acids
(Glutamic acid, Lysine).
UNIT-IV ENZYMEKINETICS 9
Overview of enzyme and its action – Time course of enzymatic reactions – Effects of substrate
concentration on velocity – Steady state model of enzyme kinetics – Significance of kcatandKm –
Experimental Measurement of kcatandKm – Linear transformations of enzyme kinetic data – Bi Bi
reaction mechanisms – Modes of reversible inhibition.
UNIT-V APPLICATIONSOFENZYMES 9
Enzymes in organic synthesis – Enzymes as biosensors – Enzymes forfood, pharmaceutical,
tannery, textile, paper and pulp industries applications – Enzyme for environmental applications –
Enzymes for analytical and diagnostic applications – Enzymes for molecular biology research.
Total Contact Hours : 45

Course Outcomes:
 Fundamentals and important parameters in fermentationprocesses
 Industrial fermentation process for enzymeproduction
 Industrially important enzymes and their use in producing biologicalmetabolites
 Enzymekinetics
 Applications of enzymes as biosensors and in other varied industrialapplications

Text Book(s):
Buchholz, K., Kasche, V. and Bornscheuer, U., “Biocatalysts and Enzyme Technology”, WILEY– VCH,
1
2005.
Mansi, E.M.T.EL., Bryce, C.F.A., Demain, A.L. and Allman, A.R., “Fermentation Microbiology and
2
Biotechnology”, Taylor and Francis,2006.

Reference Books(s) / Web links:
1 Copeland, R. A., “Enzymes”, 2 nd Edition, WILEY–VCH,2008.
2 Najafpour, G.D., “Biochemical Engineering & Biotechnology”, Elsevier,2007.
3 McNeil, B., Harvey, L., “Practical Fermentation Technology”, John Wiley & Sons,2008.

BY19103 BIOINFORMATICS AND APPLICATIONS Category L T P C
3 0 0 3

Objectives:
 To develop skill in Perl Programming and Linux commands.
 To provide knowledge on DBMS and different Biological Databases.
 To develop data analysis/visualisation skill with basic knowledge of data analysis
 To understand different algorithms of Sequence Alignment/Structural Bioinformatics
 To understand different statistical methods and application in Biology.