Page 13 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

products).
UNIT-II PHYSICAL METHODSOFSEPERATION 9
Unit operations for solid-liquid separation - Filtration at constant pressure and at constant rate – cake filtration–
Types of filtration equipment’s – Centrifugation – Basic principles, design characteristics – Types of centrifuges
and applicationsandcentrifugation.
UNIT-III ISOLATIONOFPRODUCTS 10
Theory, Design consideration and configuration of membrane separation processes – Reverse osmosis,
microfiltration, ultra filtration, dialysis and pervaporation – Structure and characteristics of membranes –
Membrane modules – Enrichment Operations – Extraction– Aqueous two-phase extraction process – Adsorption
isotherms and techniques – Protein precipitation – Methods of precipitation.
UNIT-IV PRODUCTPURIFICATION 10
Chromatography – Classification of chromatographic techniques – General description of column chromatography
– Chromatographic terms and parameters – Practice of chromatography – Partition, normal-phase, displacement,
reversed-phase, size exclusion, ion exchange, hydrophobic, affinity chromatography – Scale-up of chromatography
– Process considerations in Preparative liquid chromatography and HPLC .
UNIT-V FINAL PRODUCT FORMULATION ANDFINISHINGOPERATIONS 8
Drying – Mechanism, methods and applications, Types of dryers – Tray, spray, rotary, belt, disc –
Crystallization – Nucleation , growth – Types of crystallizers – Freeze drying – Principle, process, applications
– Case studies- Citric acid, Penicillin , cephalosporin.
Total Contact Hours : 45

Course Outcomes:
 Define the importance and fundamentals of downstream processing for productrecovery.
Understand the requirements for successful operations of downstream processing in solid liquid

separation.
 Describe the components of downstream equipment in liquid- liquidseparation.
 Apply the principles of various chromatographic techniques used in downstreamprocessing.
 Learn the mechanism and applications in finishing operations andformulations.

Text Book(s):
Belter, P.A. E.L. Cussler And Wei-Houhu – “Bioseparations – Downstream Processing or
1
Biotechnology, Wiley Interscience Pun.(1988).
2 Sivasankar, B. “Bioseparations : Principles and Techniques”. PHI,2005.
3 Ghosh, R., “Principles of Bioseparations Engineering”, World Scientific Publishers,2006.

Reference Books(s) / Web links:
R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology By Open
1
Learning Series, Butterworth-Heinemann(1992).
J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution Methods And
2
Applications, VCH Pub.1989.
3 R.K. Scopes – Protein Purification – Principles And Practice, Narosa Pub.(1994).

BY19202 BIOREACTION ENGINEERING Category L T P C
3 0 0 3

Objectives:
This course will help the students to learn about the design of immobilized enzyme reactors and various

structured and unstructuredmodels.
 To gain knowledge about recombinant cell cultivation and the design of reactors for thosecultivation.

UNIT-I METABOLIC STOICHIOMETRYANDENERGETICS 9
Mass and energy balance in biological system – Stoichiometry of cell growth and product formation – Elemental
balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of