Page 37 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

COURSE OUTCOME:
The students would visualize and interpret the theory of spectroscopic methods by hands on experiments.

REFERENCES
1. Skoog, D.A. etal. ―Principles of Instrumental Analysis‖, Vth Edition, Thomson / Brooks – Cole,1998.
2. Braun, R.D. ―Introduction to Instrumental Analysis‖, Pharma Book Syndicate, 1987.
3. Willard, H.H. etal. ―Instrumental Methods of Analysis‖, VIth Edition, CBS, 1986.
4. Ewing,G.W. ―Instrumental Methods of Chemical Analysis‖, Vth Edition, McGraw-Hill, 1985.

BT17312 CELL BIOLOGY LABORATORY L T P C
0 0 4 2

OBJECTIVES:
At the end of the course students will have in-depth knowledge about the various physiological and biological
functions of the cell.
 To learn the various techniques in microscopy
 To learn about the trans port mechanism in the cell

Experiments
1. Introduction to principles of microscopy & parts of a microscope
2. Identification of given plant, animal and bacterial cells & their components by microscopy
3. Staining techniques – Leishman, Giesma Staining
4. Separation of Peripheral Blood Mononuclear Cells from blood
5. Osmosis and Tonicity
6. Tryphan Blue Assay
7. Staining for different stages of mitosis in AlliumCepa (Onion)
8. Thin Layer Chromatography – separation of plant pigments by TLC
9. Extraction of DNA from plant sources
10. Separation of mixtures using differential & isopycnic centrifugal techniques

TOTAL: 60 PERIODS
COURSE OUTCOMES:
 To learn the principles of cell propagation and sterilization
 To become familiar about the different staining methods for the identification of microbes.
 Ability to identify the different blood cells
 To gain knowledge on cell division.

REFERENCES
1. Rickwood, D. and J.R. Harris ―Cell Biology: Essential Techniques‖, Johnwiley, 1996.
2. Davis, J.M. ―Basic Cell Culture: A Practical Approach‖, IRL, 1994.

HS17361 INTERPERSONAL AND SKILLS- LISTENING SPEAKING
Common to CSE, ECE, IT, MECH, BT, AUTO, CIVIL, MCT& BME
L T P C
0 0 2 1
OBJECTIVES
 To upgrade the learners’ listening and speaking skills for educational purposes.
 To enhance the employability skills of the learners with a special focus on listening and speaking skills.

UNIT I INTRODUCTION 6
Importance of listening and Types of Listening – listening to TED Talks, lectures, etc. Speaking: group discussions
on general topics like how to grow organic potted plants, to furnish an apartment inexpensively, etc. – Phonetics
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 37