Page 35 - B.Tech Biotech Curriculum and Syllabus R2017 - REC
P. 35
Department of BIOTECHNOLOGY, REC
UNIT V TECHNIQUES USED TO STUDY CELLS 9
Cell fractionation and flow cytometry, Morphology and identification of cells using microscopic studies like
SEM, TEM, Fluorescent and Confocal Microscopy. Localization of proteins in cells – Immunostaining.
TOTAL : 45 PERIODS
COURSE OUTCOMES:
At the end of the course the students will be able to
apply knowledge in the area of cellular organisation
analyse the modes of transport and relate the same to function of nervous system
examine the methods of communication within the cells
correlate the basic concepts of cell division and their role in carcinogenesis
design simple methods to analyse cell and their morphology
TEXT BOOKS
1. Lodish, Harvey etal., ―Molecular Cell Biology‖, Vth Edition, W.H.Freeman, 2005.
2. Cooper, G.M. and R.E. Hansman ―The Cell : A Molecular Approach‖, IVth Edition, ASM Press,
2007.
REFERENCES
th
1. Lehninger A.L., Nelson D.L. and Cox M.M. Principles of Biochemistry,6 edition, CBS publishers
and distributors, 2012.
2. LubertStryer W.H. Biochemistry, 5 Revised edition Freeman and company, New York, 2002.
th
BT17305 STOICHIOMETRY AND FLUID MECHANICS L T P C
3 2 0 4
OBJECTIVES:
This course aims at imparting knowledge about the basics of stoichiometric calculations, material and
energy balances
The students will learn the basic knowledge about the properties of fluids and fluid dynamics.
UNIT I INTRODUCTION 12
Dimensions – system of units - conversion factors - Compositions of mixtures and solutions - gas laws-
humidity
UNIT II CONCEPTS IN MATERIAL BALANCES 12
Material balance concept – overall & component – material balance applications for evaporator, gas absorber
without reaction, Distillation (Binary system), Liquid extraction, solid-liquid extraction, crystallization,
drying, Humidification, Reverse Osmosis separation and Mixing Recycle and Bypass illustration. Chemical
Reaction-Limiting, excess component, Fractional conversion, Percent conversion, Fractional yield-
Combustion Reactions.
UNIT III CONCEPTS IN ENERGY BALANCES 12
General energy balance equation for open systems, closed system sensible heat calculation, Heat required for
phase change - thermo chemistry - Application of energy balance in Bioprocesses
UNIT IV FLUID PROPERTIES & FLUID MECHANICS 12
Fluid definition- fluid statics and its application – Boundary layer – Separation and wake formation - Fluid
Dynamics – equation of continuity – Bernoulli‘s equation – Flow of incompressible fluids in pipes - Fluid
flow measurement, Orifice, venturi and Rotameter.
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 35

